Identification of genes controlled by quorum sensing in Pseudomonas aeruginosa
Identification of genes controlled by quorum sensing in Pseudomonas aeruginosa
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DOI：
10.1073/pnas.96.24.13904
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发表时间：
1999-11-23
影响因子：
11.1
通讯作者：
Greenberg, EP
中科院分区：
文献类型：
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作者：
Whiteley, M;Lee, KM;Greenberg, EP
细菌之间相互通信，以细胞密度依赖的方式协调特定基因的表达，这种现象被称为群体感应和反应。虽然我们知道群体感应通过酰基高丝氨酸内酯(HSL)信号控制了几个毒力基因在人类病原菌铜绿假单胞菌中的表达，但群体感应控制的基因的数量和类型还没有得到系统的研究。我们利用含有无启动子LacZ的转座子，构建了铜绿假单胞菌acyl-HSL合成突变体的染色体随机插入文库。该文库经Far-acyl-HSL诱导LacZ筛选。共鉴定出39个群体感应调控基因。根据调控模式，这些基因被组织成不同的类别。大约一半的基因似乎存在于七个操纵子中，其中一些似乎是在基因组的目标补丁中组织起来的。许多受群体感应调节的基因编码假定的毒力因子或次生代谢物的产生。许多已鉴定的基因都显示了酰基-HSL信号的高水平诱导。
Bacteria communicate with each other to coordinate expression of specific genes in a cell density-dependent fashion, a phenomenon called quorum sensing and response. Although we know that quorum sensing via acyl-homoserine lactone (HSL) signals controls expression of several virulence genes in the human pathogen Pseudomonas aeruginosa, the number and types of genes controlled by quorum sensing have not been studied systematically. We have constructed a library of random insertions in the chromosome of a P. aeruginosa acyl-HSL synthesis mutant by using a transposon containing a promoterless lacZ. This library was screened far acyl-HSL induction of lacZ. Thirty-nine quorum sensing-regulated genes were identified. The genes were organized into classes depending an the pattern of regulation. About half of the genes appear to be in seven operons, some seem organized in targe patches on the genome. Many of the quorum sensing-regulated genes code for putative virulence factors or production of secondary metabolites. Many of the genes identified showed a high level of induction by acyl-HSL signaling.