Evaluation of interleukin -1B (+3954) gene polymorphism in patients with chronic and aggressive periodontitis: A genetic association study.
Evaluation of interleukin -1B (+3954) gene polymorphism in patients with chronic and aggressive periodontitis: A genetic association study.
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DOI：
10.4103/0976-237x.96815
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发表时间：
2012-04
影响因子：
1.2
通讯作者：
Bhat K
中科院分区：
文献类型：
--
作者：
Masamatti SS;Kumar A;Baron TK;Mehta DS;Bhat K
IL-1细胞因子在牙周病的发病机制中起核心作用。人类IL-1B基因+3954位点（C/T）的多态性已被证明会影响这种细胞因子的水平。本研究的目的是探讨IL-1 B（+3954）基因多态性与不同临床形式牙周炎发生之间的关系。从90个个体中获得基因组DNA，并利用IL-1B +3954位点侧的特异性引物进行PCR扩增。PCR产物经限制性内切酶酶切和凝胶电泳分析，确定基因型和多态性检测。采用Fisher精确检验比较组间基因型分布的频率。慢性牙周炎组的T等位基因比例（38%）高于侵袭性牙周炎组（20%）和对照组（19%）。我们的研究数据表明，IL-1B基因+3954位点的多态性可能是卡纳塔克邦印度人群样本中慢性牙周炎的危险因素。
IL-1 cytokines have central roles in the pathogenesis of periodontal disease. Polymorphism in the locus +3954 (C/T) of the human IL-1B gene has been shown to affect the levels of this cytokine. The aim of the present study was to investigate the association between the IL-1 B (+3954) gene polymorphism and the occurrence of different clinical forms of periodontitis. Genomic DNA was obtained from 90 individuals and amplified using the PCR with specific primers flanking the locus +3954 of IL-1B. PCR products were submitted to restriction endonuclease digestion and analyzed by gel electrophoresis, allowing for the determination of the genotypes and detection of the polymorphism. Fisher's exact test was used for comparing the frequency of genotype distributions between groups. The chronic periodontitis group displayed a higher percentage of T alleles (38%) when compared to the aggressive periodontitis group (20%) and to the control group (19%). Our study data states that polymorphism in the locus +3954 of IL-1B gene could be a risk factor for chronic periodontitis in a sample of Indian population of Karnataka state.