Integrative Analysis of Terpenoid Profiles and Hormones from Fruits of Red-Flesh Citrus Mutants and Their Wild Types
Integrative Analysis of Terpenoid Profiles and Hormones from Fruits of Red-Flesh Citrus Mutants and Their Wild Types
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红肉柑橘突变体及其野生型果实中萜类化合物和激素的综合分析
DOI：
10.3390/molecules24193456
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发表时间：
2019-10-01
期刊：
影响因子：
4.6
通讯作者：
Xu, Juan
中科院分区：
文献类型：
--
作者：
Liu, Cuihua;He, Min;Xu, Juan
在柑橘颜色突变体中，类胡萝卜素成分和其他次生代谢产物的水平在其相应的野生型中是不同的。萜类化合物与着色、苦味和风味密切相关。本研究采用GC/MS、HPLC和LC-MS/MS等技术对红安柳橙子和红肉关西柚两个红肉突变体及其野生型果实中萜类化合物和激素进行了分析。结果表明，红安柳橙和红肉关西柚果实中萜类化合物的含量均高于野生型橙子（类胡萝卜素含量高）和安柳橙子（类胡萝卜素含量低）积累了低水平的柠檬酸苷元，但积累了高水平的单萜类化合物;相反地，红肉型（类胡萝卜素含量高）和类胡萝卜素缺乏型（类胡萝卜素缺乏）的关西柚积累了高水平的柠檬酸苷元，但低水平的单萜类化合物。然而，异戊烯基二磷酸以相似的水平存在。相关性分析表明，茉莉酸和水杨酸可能在调节萜类化合物生物合成中起重要作用。此外，类胡萝卜素和挥发物的模式之间的每个突变体和相应的野生型的相似性大于那些之间的两个突变体或两个野生型。柑橘类萜类化合物代谢的通量平衡在具有不同萜类化合物谱的柑橘属间趋于稳定。芽体突变对柑橘果实代谢产物的影响有限。
In citrus color mutants, the levels of carotenoid constituents and other secondary metabolites are different in their corresponding wild types. Terpenoids are closely related to coloration, bitterness, and flavor. In this study, terpenoid profiles and hormones in citrus fruits of two red-flesh mutants—Red Anliu orange and Red-flesh Guanxi pummelo—and their corresponding wild types were investigated using GC/MS, HPLC, and LC-MS/MS. Results showed that Red Anliu orange (high in carotenoids) and Anliu orange (low in carotenoids) accumulated low levels of limonoid aglycones but high levels of monoterpenoids; conversely, Red-flesh Guanxi pummelo (high in carotenoids) and Guanxi pummelo (deficient in carotenoids) accumulated high levels of limonoid aglycones but low levels of monoterpenoids. However, isopentenyl diphosphate was present at similar levels. A correlation analysis indicated that jasmonic and salicylic acids might play important roles in regulating terpenoid biosynthesis. Additionally, the similarities of carotenoid and volatile profiles between each mutant and its corresponding wild type were greater than those between the two mutants or the two wild types. The flux balance of terpenoid metabolism in citrus fruit tends toward stability among various citrus genera that have different terpenoid profiles. Bud mutations could influence metabolite profiles of citrus fruit to a limited extent.