Monocyte-derived CD1a+ dendritic cells generated in two different culture systems: immunophenotypic and functional comparison.
Monocyte-derived CD1a+ dendritic cells generated in two different culture systems: immunophenotypic and functional comparison.
复制标题
在两种不同的培养系统中产生的单核细胞衍生的 CD1a 树突状细胞：免疫表型和功能比较。
DOI：
10.1046/j.1365-3083.2003.01238.x
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发表时间：
2003
影响因子：
3.7
通讯作者：
Kao,KJ
中科院分区：
文献类型：
--
作者：
Xia,C-Q;Kao,KJ
以往的研究表明，CD 1a+树突状细胞（DCs）不能在不使用胎牛血清（FCS）的情况下被活化。最近，我们开发了一种方法，使用肝素诱导人单核细胞分化为CD 1a +DCs，而不使用FCS。为了确定肝素诱导的CD 1a +DCs的潜在临床适用性，我们进行了这项研究，以比较两种类型的CD 1a +DCs的免疫表型和功能。我们的结果表明，肝素-DC上的CD 1a表达低于FCS-DC上的CD 1a表达。两种类型的DC在脂多糖刺激前后表达相似水平的CD 11 c、HLA-DR、CD 40、CD 83、CD 80和CD 86。未成熟的肝素-DC和FCS-DC具有相似的吞噬活性。活化后，肝素-DC始终比FCS-DC分泌更高的白细胞介素-10（IL-10）和更少的IL-12。在刺激同种异体CD 4 +T细胞增殖方面，成熟肝素-DC比成熟FCS-DC稍有活性。两种类型的成熟CD 1a +DCs均引发幼稚CD 4 +T细胞产生大量干扰素-γ（IFN-γ）。然而，用FCS-DC刺激的幼稚CD 4 +T细胞产生更多的IFN-γ，而用肝素-DC刺激的幼稚CD 4 +T细胞产生更多的IL-5。结果表明，两种类型的CD 1a + DC在CD 4 +T细胞的启动中的功能并不完全相同，并且在免疫表型上也有微小的差异。
Previous studies demonstrated that CD1a+dendritic cells (DCs) could not be preparedex vivowithout using fetal calf serum (FCS). Recently, we developed a method of using heparin to induce differentiation of human monocytes into CD1a+DCs without using FCS. In order to determine the potential clinical applicability of heparin‐induced CD1a+DCs, we conducted this study to compare both types of CD1a+DCs, immunophenotypically and functionally. Our results showed that the expression of CD1a on heparin‐DCs was lower than that on FCS‐DCs. Both types of DCs expressed similar levels of CD11c, HLA‐DR, CD40, CD83, CD80 and CD86 before and after lipopolysaccharide stimulation. Immature heparin‐DCs and FCS‐DCs had similar phagocytic activities. Heparin‐DCs consistently secreted higher interleukin‐10 (IL‐10) and lesser IL‐12 than FCS‐DCs after activation. Mature heparin‐DCs were slightly more active than mature FCS‐DCs in stimulating the proliferation of allogeneic CD4+T cells. Both types of mature CD1a+DCs primed the naïve CD4+T cells to produce large amount of interferon‐γ (IFN‐γ). However, naïve CD4+T cells stimulated with FCS‐DCs produced more IFN‐γ, while the naïve CD4+T cells stimulated with heparin‐DCs produced more IL‐5. The results indicate that both types of CD1a+DCs do not have identical function in the priming of CD4+T cells and have minor difference in immunophenotypes.