The loss of phenotypic traits by differentiated cells. 3. The reversible behavior of chondrocytes in primary cultures.
The loss of phenotypic traits by differentiated cells. 3. The reversible behavior of chondrocytes in primary cultures.
复制标题
分化细胞的表型性状丧失。 3。软骨细胞在原发性培养物中的可逆行为。
DOI：
10.1083/jcb.28.3.473
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发表时间：
1966-03
影响因子：
7.8
通讯作者：
Holtzer, H
中科院分区：
文献类型：
--
作者：
Abbott, J;Holtzer, H
观察软骨细胞在不同条件下的体外生长行为。10天鸡胚椎骨中的软骨细胞作为完整椎骨的培养物生长，作为从其基质中释放的软骨细胞的小球，以及作为在血浆凝块上铺板的单分散细胞。甲苯胺蓝异染软骨基质。放射自显影由掺入的H3-胸苷、H3-脯氨酸和S35-硫酸盐制成，以分别确定DNA合成、胶原合成和硫酸软骨素合成的程度。完整椎骨或颗粒中的软骨细胞呈圆形，并积极合成硫酸软骨素和胶原蛋白。无论是椎骨还是小球中的细胞都几乎没有DNA合成。作为单分散细胞生长的软骨细胞迅速停止合成细胞学可检测的硫酸软骨素，并被诱导合成DNA和分裂。这些软骨细胞的形状发生变化，从圆形变为星状，这伴随着代谢活动的变化。相反，当细胞达到一定的细胞密度时，它们重新获得圆形形状，停止分裂，并再次合成硫酸软骨素。成簇的软骨细胞比分离的软骨细胞合成更多的硫酸软骨素。可以得出结论，大多数软骨细胞合成硫酸软骨素不同时合成DNA。相关软骨细胞之间的相互作用在诱导和维持遗传决定的软骨细胞中的硫酸软骨素合成中是重要的。软骨细胞之间相互作用的失败导致DNA合成和细胞增殖。
Observations were made on the behavior of chondrocytes grown under various conditions in vitro. The chondrocytes in 10-day embryonic chick vertebrae were grown as cultures of intact vertebrae, as pellets of chondrocytes liberated from their matrix, and as monodispersed cells plated out on plasma clots. Cartilage matrix was stained metachromatically with toluidine blue. Radioautographs were made of incorporated H3-thymidine, H3-proline, and S35-sulfate to determine the extent of DNA synthesis, collagen synthesis, and chondroitin sulfate synthesis, respectively. Chondrocytes in intact vertebrae or in pellets are rounded and actively synthesizing chondroitin sulfate and collagen. There is little DNA synthesis by cells in either vertebrae or pellets. Chondrocytes grown as monodisperse cells rapidly cease synthesizing cytologically detectable chondroitin sulfate and are induced to synthesize DNA and divide. There is a change in the shape of these chondrocytes from a rounded to a more stellate condition which accompanies the shift in metabolic activity. Conversely, when the cells attain a certain cell density, they reacquire a rounded shape, cease dividing, and again synthesize chondroitin sulfate. Clusters of chondrocytes synthesize more chondroitin sulfate than isolated chondrocytes. It is concluded that most chondrocytes synthesizing chondroitin sulfate do not concurrently synthesize DNA. Interaction between associated chondrocytes is important in inducing and maintaining chondroitin sulfate synthesis in genetically determined chondrocytes. Failure of interaction between chondrocytes leads to DNA synthesis and cell multiplication.