Reducing the stress of drug administration: implications for the 3Rs
Reducing the stress of drug administration: implications for the 3Rs
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DOI：
10.1038/srep14288
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发表时间：
2015-09-23
影响因子：
4.6
通讯作者：
Robinson, Emma S. J.
中科院分区：
文献类型：
--
作者：
Stuart, Sarah A.;Robinson, Emma S. J.
众所周知，动物的束缚会造成压力，但在啮齿动物的几乎所有科学程序中都会使用束缚，这代表了一个重大的福利和科学问题。物质管理是大多数科学程序的关键部分，几乎总是涉及到对动物的身体约束。在这项研究中，我们开发了一种使用非限制性技术向大鼠注射物质的方法。然后，我们比较了限制性注射和非限制性注射对生理、行为和情绪的影响。我们的结果突出了与身体约束相关的负面福利影响，并展示了一种可以用来避免这种情况的方法。我们的工作表明，采取避免约束的策略可以最大限度地减少实验动物中广泛存在的压力源，并通过改进来改善福利。
Restraint in animals is known to cause stress but is used during almost all scientific procedures in rodents, representing a major welfare and scientific issue. Administration of substances, a key part of most scientific procedures, almost always involves physical restraint of the animal. In this study, we developed a method to inject substances to rats using a non-restrained technique. We then compared the physiological, behavioral and emotional impacts of restrained versus non-restrained injection procedures. Our results highlight the negative welfare implications associated with physical restraint and demonstrate a method which can be used to avoid this. Our work shows how adopting strategies that avoid restraint can minimize a widespread source of stress in laboratory animals and improve welfare through refinement.