Chicken TBK1 interacts with STING and is involved in IFN-β signaling regulation
Chicken TBK1 interacts with STING and is involved in IFN-β signaling regulation
复制标题
鸡 TBK1 与 STING 相互作用并参与 IFN-β 信号传导调节
DOI：
10.1016/j.dci.2017.08.011
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发表时间：
2017-12-01
影响因子：
2.9
通讯作者：
Sun, Jianhe
中科院分区：
文献类型：
--
作者：
Cheng, Yuqiang;Ma, Jingjiao;Sun, Jianhe
TANK-binding kinase 1 (TBK1) is an essential serine/threonine-protein kinase required for the Toll-like receptor (TLR)- and retinoic acid-inducible gene I (RIG-I) -mediated induction of type I IFN. Through endogenous Co-IP and LC-MS/MS, we identified chicken TBK1 (chTBK1) as a chSTING-interactive protein. Through exogenous Co-IP assay in transfected cells, we confirmed the interaction between chSTING and chTBK1. To better understand the biological role of chTBK1 in the chSTING-mediated IFN pathway, we cloned the chTBK1 and investigated its biological functions. Quantitative RT-PCR showed that chTBK1 mRNA was widely expressed in different tissues. The overexpression of chTBK1 in DF-1 cells induced the expression of IFN-beta and ISGs and inhibited AIV viral replication. We identified indispensable domains of chTBK1 on IFN-beta production via the generation of various chTBK1 mutant forms. Together, we identified the chTBK1 as a chSTING interactive protein and concluded that chTBK1 is involved in chSTING-triggered IFN-beta signaling in chicken cells. (C) 2017 Elsevier Ltd. All rights reserved.
