Zinc-releasing calcium phosphate for stimulating bone formation
Zinc-releasing calcium phosphate for stimulating bone formation
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DOI：
10.1016/s0928-4931(02)00108-x
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发表时间：
2002-10-01
期刊：
影响因子：
--
通讯作者：
Ichinose, N
中科院分区：
文献类型：
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作者：
Ito, A;Kawamura, H;Ichinose, N
含锌磷酸三钙（ZnTCP）具有生物相容性和生物活性，是一种有效的锌载体。ZnTCP最多含有12摩尔%的锌。采用1100℃烧结法制备了(Ca+Zn)/P摩尔比为1.60的ZnTCP陶瓷和ZnTCP与羟基磷灰石复合陶瓷（ZnTCP/HAP）。ZnTCP/HAP在0.9% wt.%氯化钠溶液中持续释放锌50天以上。锌含量为1.2 wt.%的ZnTCP/HAP能显著提高体外培养大鼠成骨细胞MC3T3-E1的增殖能力和基质细胞碱性磷酸酶活性。与不含锌的对照组相比，锌含量为0.316 wt.%的ZnTCP/HAP在植入4周后使兔骨形成增加51% （n = 6; p = 0.0509）。锌浓度在溶液的非细胞毒性水平内不会完全阻断溶液中磷灰石的晶体生长。磷酸钙水泥中加入ZnTCP后，对水泥的凝结能力既没有抑制作用，也没有促进作用。这些结果表明，含锌磷酸三钙是一种具有促进骨形成的药物作用的生物材料。(C) 2002 Elsevier Science B.V.版权所有
Zinc-containing tricalcium phosphate (ZnTCP) is biocompatible and bioactive, and functions as an effective zinc carrier. ZnTCP contains a maximum of 12 mol% of zinc. ZnTCP ceramics and composite ceramics of ZnTCP and hydroxyapatite (ZnTCP/HAP) with a (Ca+Zn)/P molar ratio of 1.60 were prepared by sintering at 1100 degreesC. ZnTCP/HAP continued to release zinc for more than 50 days in 0.9 wt.% sodium chloride solution. ZnTCP/HAP with a zinc content of 1.2 wt.% significantly increased osteoblastic MC3T3-E1 cell proliferation and alkaline phosphatase activity of rat stromal cells in vitro. ZnTCP/HAP with a zinc content of 0.316 wt.% increased bone formation by 51% (n = 6; p = 0.0509) 4 weeks after implantation in rabbit compared to the control without zinc. A zinc concentration within a noncytotoxic level of a solution does not completely block the crystal growth of apatite in the solution. When ZnTCP is added to calcium phosphate cement, the ZnTCP showed neither inhibitory nor promoting effect on the setting ability of the cement. All these findings suggest that the zinc-containing tricalcium phosphate is a biomaterial that has a pharmaceutical effect of promoting bone formation. (C) 2002 Elsevier Science B.V. All rights reserved.