The WRKY57 Transcription Factor Affects the Expression of Jasmonate ZIM-Domain Genes Transcriptionally to Compromise Botrytis cinerea Resistance
The WRKY57 Transcription Factor Affects the Expression of Jasmonate ZIM-Domain Genes Transcriptionally to Compromise Botrytis cinerea Resistance
复制标题
WRKY57 转录因子通过转录影响茉莉酸 ZIM 结构域基因的表达，从而损害灰霉病抗性
DOI：
10.1104/pp.16.00747
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发表时间：
2016-08-01
期刊：
影响因子：
7.4
通讯作者：
Yu, Diqiu
中科院分区：
文献类型：
--
作者：
Jiang, Yanjuan;Yu, Diqiu
虽然坏死性病原体引起许多毁灭性的植物疾病，但我们对植物防御反应的了解是有限的。本研究发现，WRKY57功能缺失增强了拟南芥（Arabidopsis thaliana）对灰霉病菌（Botrytis cinerea）感染的抗性。进一步研究表明，WRKY57对葡萄球菌的负调控作用依赖于茉莉酸（jasmonic acid， JA）信号通路。染色质免疫沉淀实验显示，WRKY57直接结合JASMONATE ZIM-DOMAIN1 （JAZ1）和JAZ5的启动子，编码JA信号通路的两个重要抑制因子，并激活它们的转录。体内和体外实验表明，WRKY57与核编码的SIGMA因子结合蛋白1 （SIB1）和SIB2相互作用。进一步的实验表明，SIB1和SIB2的相同结构域VQ基序与WRKY33和WRKY57相互作用。此外，瞬时转录活性分析证实WRKY57和WRKY33竞争性调控JAZ1和JAZ5， SIB1和SIB2进一步增强了WRKY57对WRKY33的竞争性。因此，转录激活因子和转录抑制因子协同调节拟南芥对灰孢杆菌的抗性，有利于植物的防御。
Although necrotrophic pathogens cause many devastating plant diseases, our understanding of the plant defense response to them is limited. Here, we found that loss of function of WRKY57 enhanced the resistance of Arabidopsis (Arabidopsis thaliana) against Botrytis cinerea infection. Further investigation suggested that the negative regulation of WRKY57 against B. cinerea depends on the jasmonic acid (JA) signaling pathway. Chromatin immunoprecipitation experiments revealed that WRKY57 directly binds to the promoters of JASMONATE ZIM-DOMAIN1 (JAZ1) and JAZ5, encoding two important repressors of the JA signaling pathway, and activates their transcription. In vivo and in vitro experiments demonstrated that WRKY57 interacts with nuclear-encoded SIGMA FACTOR BINDING PROTEIN1 (SIB1) and SIB2. Further experiments display that the same domain, the VQ motif, of SIB1 and SIB2 interact with WRKY33 and WRKY57. Moreover, transient transcriptional activity assays confirmed that WRKY57 and WRKY33 competitively regulate JAZ1 and JAZ5, SIB1 and SIB2 further enhance these competitions of WRKY57 to WRKY33. Therefore, coordinated regulation of Arabidopsis against B. cinerea by transcription activators and repressors would benefit plants by allowing fine regulation of defense.