Effects of enhanced O(2) transport on hepatocytes packed within a bioartificial liver device.
Effects of enhanced O(2) transport on hepatocytes packed within a bioartificial liver device.
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增强的 O(2) 运输对生物人工肝装置内肝细胞的影响。
DOI：
10.1089/107632704322791899
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Coger,RobinN
中科院分区：
文献类型：
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作者：
McClelland,RandallE;Coger,RobinN
体外生物人工肝（BAL）装置的功能性能要求存在合适的营养途径以支持包装在其中的肝细胞。 营养氧的距离是许多BAL设计放大的限制因素。在这项研究中，胶原蛋白细胞外基质的孔隙率增加，以评估如何增强O2转运改变的活力和功能性能的凝胶包埋的肝细胞包装在一个BAL。我们的研究结果表明，多孔胶原蛋白增加了活肝细胞的数量， 可由单一O2源支持。此外，结果表明，与正常胶原相比，多孔胶原延长了O2运输距离，使得肝细胞 位于离BAL的O2源更远的位置。最后，功能结果揭示，多孔胶原蛋白内的肝细胞经历明显改善 在对照培养物上起作用。因此，我们的研究结果表明，通过密集包装的BAL设计的细胞外基质增强O2转运是显著改善 这些设备的功能。
The functional performance of an extracorporeal bioartificial liver (BAL) device requires that suitable nutrient pathways exist to support the hepatocytes packed within it. Consequently the limited transport distance of the nutrient oxygen is a limiting factor in the scale-up of many BAL designs. In this study the porosity of a collagen extracellular matrix is increased to evaluate how enhanced O2transport alters the viability and functional performance of gel-entrapped hepatocytes packed within a BAL. Our results indicate that the porous collagen increases the number of viable hepatoctyes that can be supported by a single O2source. Furthermore, the results illustrate that, compared with normal collagen, porous collagen extends the O2transport distance such that hepatocytes located at larger distances from the O2source of the BAL can be supported. Finally, the function results reveal that hepatocytes within the porous collagen experience significantly improved function over the control cultures. Hence our results demonstrate that enhancing O2transport through the extracellular matrix of densely packed BAL designs is one way to significantly improve the functionality of these devices.