Translation mechanisms involving long-distance base pairing interactions between the 5′ and 3′ non-translated regions and internal ribosomal entry are conserved for both genomic RNAs of Blackcurrant reversion nepovirus
Translation mechanisms involving long-distance base pairing interactions between the 5′ and 3′ non-translated regions and internal ribosomal entry are conserved for both genomic RNAs of Blackcurrant reversion nepovirus
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DOI：
10.1016/j.virol.2007.10.003
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发表时间：
2008-02-20
期刊：
影响因子：
3.7
通讯作者：
Lehto, Kirsi
中科院分区：
文献类型：
--
作者：
Karetnikov, Alexey;Lehto, Kirsi
位于3‘非翻译区（NTRs）的病毒帽独立翻译增强子（CITEs）的功能机制之一是3’ NTR-5'前导体长距离碱基配对。在此之前，我们已经证明黑加仑逆转录病毒（BRV）的RNA2 - 3' NTR包含一个CITE，该CITE必须与5' NTR碱基对以促进翻译。本文通过诱变植物原生质体中萤火虫荧光素酶报告基因mRNA中的BRV NTRs，比较了BRV RNAI和RNA2的翻译策略。基于3' CITEs、5' NTR-3' NTR碱基配对和poly(A) tail刺激的翻译机制在RNA1和RNA2之间具有保守性。40S核糖体亚基至少部分通过内部核糖体进入位点（IRES）进入RNAI先导区。两个与植物18S rRNA互补的RNAI先导片段增强翻译。本文讨论了BRV rna的翻译模型，包括mes依赖的40S亚基招募和长距离的5' NTR-3' NTR碱基配对。(C) 2007爱思唯尔公司版权所有。
One of the mechanisms of functioning for viral cap-independent translational enhancers (CITEs), located in 3' non-translated regions (NTRs), is 3' NTR-5' leader long-distance base pairing. Previously, we have demonstrated that the RNA2 3' NTR of Blackcurrant reversion nepovirus (BRV) contains a CITE, which must base pair with the 5' NTR to facilitate translation. Here we compared translation strategies employed by BRV RNAI and RNA2, by using mutagenesis of the BRV NTRs in firefly luciferase reporter mRNA, in plant protoplasts. Translation mechanisms, based on 3' CITEs, 5' NTR-3' NTR base pairing and poly(A) tail-stimulation, were found conserved between RNA1 and RNA2. The 40S ribosomal subunit entry at the RNAI leader occurred, at least partly, via an internal ribosomal entry site (IRES). Two RNAI leader segments complementary to plant 18S rRNA enhanced translation. A model for BRV RNAs translation, involving MES-dependent 40S subunit recruitment and long-distance 5' NTR-3' NTR base pairing, is discussed. (C) 2007 Elsevier Inc. All rights reserved.