Tracheal reconstruction using chondrocytes seeded on a poly(L-lactic-co-glycolic acid)-fibrin/hyaluronan
Tracheal reconstruction using chondrocytes seeded on a poly(L-lactic-co-glycolic acid)-fibrin/hyaluronan
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DOI：
10.1002/jbm.a.35091
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发表时间：
2014-11-01
影响因子：
4.9
通讯作者：
Choi, Eun Chang
中科院分区：
文献类型：
--
作者：
Hong, Hyun Jun;Chang, Jae Won;Choi, Eun Chang
气管重建至今仍是临床难题。组织工程是解决这一问题的一个新的和有前途的概念。本研究旨在探讨同种异体软骨细胞与纤维蛋白/透明质酸（HA）水凝胶和可降解多孔聚乳酸-羟基乙酸共聚物（PLGA）支架复合培养用于气管部分重建的可行性。将来自兔关节软骨的软骨细胞用纤维蛋白/HA水凝胶扩增和培养，并注射到5 × 10 mm大小的弯曲片状PLGA支架中。体外培养4周后，将支架植入8只家兔气管缺损处。术后6周和10周，通过支气管镜、放射学和组织学分析对植入部位进行评价。还评价了再生上皮的纤毛搏动频率（CBF）。8只家兔均未出现任何呼吸窘迫体征。支气管镜检查未发现重建气管狭窄，缺损完全恢复，呼吸上皮。计算机断层扫描显示气管腔轮廓良好。组织学数据显示植入的软骨细胞成功地形成了具有最少肉芽组织的新软骨。再生上皮的CBF与正常上皮相似。同种异体软骨细胞与PLGA-纤维蛋白/HA复合支架复合培养成功地重建了气管部分缺损。(C)2014 Wiley Periodicals，Inc.
Reconstruction of trachea is still a clinical dilemma. Tissue engineering is a recent and promising concept to resolve this problem. This study evaluated the feasibility of allogeneic chondrocytes cultured with fibrin/hyaluronic acid (HA) hydrogel and degradable porous poly(L-lactic-co-glycolic acid) (PLGA) scaffold for partial tracheal reconstruction. Chondrocytes from rabbit articular cartilage were expanded and cultured with fibrin/HA hydrogel and injected into a 5 x 10 mm-sized, curved patch-shape PLGA scaffold. After 4 weeks in vitro culture, the scaffold was implanted on a tracheal defect in eight rabbits. Six and 10 weeks postoperatively, the implanted sites were evaluated by bronchoscope and radiologic and histologic analyses. Ciliary beat frequency (CBF) of regenerated epithelium was also evaluated. None of the eight rabbits showed any sign of respiratory distress. Bronchoscopic examination did not reveal stenosis of the reconstructed trachea and the defects were completely recovered with respiratory epithelium. Computed tomography scan showed good luminal contour of trachea. Histologic data showed that the implanted chondrocytes successfully formed neocartilage with minimal granulation tissue. CBF of regenerated epithelium was similar to that of normal epithelium. Partial tracheal defect was successfully reconstructed anatomically and functionally using allogeneic chondrocytes cultured with PLGA-fibrin/HA composite scaffold. (C) 2014 Wiley Periodicals, Inc.