Molecular and genetic features of a labeled class of spinal substantia gelatinosa neurons in a transgenic mouse
Molecular and genetic features of a labeled class of spinal substantia gelatinosa neurons in a transgenic mouse
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DOI：
10.1002/cne.20709
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发表时间：
2005-11-07
影响因子：
2.5
通讯作者：
Perl, ER
中科院分区：
文献类型：
--
作者：
Hantman, AW;Perl, ER
在小鼠中基因整合一个包含朊病毒启动子和绿色荧光蛋白（GFP）编码序列的转基因，标记了一组在形态、电生理学和γ - 氨基丁酸表达上均一的胶状质（SG）神经元（SG - GFP）。在本次分析中，确定SG - GFP神经元具有蛋白激酶C - β II免疫反应性，且没有钙结合蛋白D - 28k、小白蛋白和蛋白激酶C - γ存在的证据。这些神经元被下行控制的介质去甲肾上腺素和5 - 羟色胺超极化。连续聚合酶链反应确定转基因插入在受体蛋白酪氨酸磷酸酶κ（RPTP - K）和层粘连蛋白受体1（核糖体蛋白SA）假基因1位点。在GFP标记的背根神经节和SG神经元中RPTP - K的表达增加了一种可能性，即RPTP - K的同嗜性相互作用有助于特定类别初级传入神经元和SG神经元之间连接的建立。
Genetic incorporation in a mouse of a transgene containing the prion promoter and the green fluorescent protein (GFP) coding sequence labels a set of substantia gelatinosa (SG) neurons (SG-GFP) homogenous in morphology, electrophysiology, and gamma-amino-butyric acid expression. In the present analysis the SG-GFP neurons are established to have protein kinase C-beta II immunoreactivity and to lack evidence for the presence of calbindin D-28k, parvalbumin, and protein kinase C-gamma. These neurons were hyperpolarized by mediators of descending control, norepinephrine and serotonin. Sequential polymerase chain reactions established the insertion of the transgene to be in the receptor protein tyrosine phosphatase kappa (RPTP-K) and the laminin receptor 1 (ribosomal protein SA) pseudogene 1 locus. RPTP-K expression in both GFP-labeled dorsal root ganglia and SG neurons raises the possibility that homophilic interactions of RPTP-K contribute to establishment of connections between specific classes of primary afferent and SG neurons.