Identification of trans-acting factors regulating SamDC expression in Oryza sativa
Identification of trans-acting factors regulating SamDC expression in Oryza sativa
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DOI：
10.1016/j.bbrc.2014.02.004
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发表时间：
2014-03-07
影响因子：
3.1
通讯作者：
Sengupta, Dibyendu N.
中科院分区：
文献类型：
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作者：
Basu, Supratim;Roychoudhury, Aryadeep;Sengupta, Dibyendu N.
非生物胁迫影响作物的生长和生产力；为了应对不利的环境条件，植物开发了有效的防御机制，其中包括酚类和类黄酮等抗氧化剂以及多胺等渗透剂。 SamDC 是植物多胺生物合成途径中的关键酶。在我们目前的通讯中，我们对 SamDC 的启动子区域进行了硅胶分析，以寻找有助于其表达的不同顺式调控元件的存在。基于不同顺式调控元件的存在，我们通过 qPCR 完成了 IR-29 和 Nonabokra 水稻叶片 SamDC 基因在盐度、干旱、寒冷非生物胁迫处理和 ABA 和光生物胁迫处理下表达的比较分析。此外，为了探讨顺式调控元件在调节植物 SamDC 基因表达中的作用，我们比较分析了经不同胁迫处理的 IR-29 和 Nonabokra 制备的水稻核蛋白的结合。与 Nonabokra 相比，IR-29 中形成的复合物的强度较低且可诱导。西南印迹分析有助于预测与这些顺式元件结合的反式作用因子的大小。据我们所知，这是第一份全面分析水稻 SamDC 基因表达并鉴定调节其表达的反式作用因子的报告。 (C) 2014 Elsevier Inc. 保留所有权利。
Abiotic stress affects the growth and productivity of crop plants; to cope with the adverse environmental conditions, plants have developed efficient defense machinery comprising of antioxidants like phenolics and flavonoids, and osmolytes like polyamines. SamDC is a key enzyme in the polyamine biosynthesis pathway in plants. In our present communication we have done in silica analysis of the promoter region of SamDC to look for the presence of different cis-regulatory elements contributing to its expression. Based on the presence of different cis-regulatory elements we completed comparative analysis of SamDC gene expression in rice lamina of IR-29 and Nonabokra by qPCR in response to the abiotic stress treatments of salinity, drought, cold and the biotic stress treatments of ABA and light. Additionally, to explore the role of the cis-regulatory elements in regulating the expression of SamDC gene in plants we comparatively analyzed the binding of rice nuclear proteins prepared from IR-29 and Nonabokra undergoing various stress treatments. The intensity of the complex formed was low and inducible in IR-29 in contrast to Nonabokra. Southwestern blot analysis helped in predicting the size of the transacting factors binding to these cis-elements. To our knowledge this is the first report on the comprehensive analysis of SamDC gene expression in rice and identification of the trans-acting factors regulating its expression. (C) 2014 Elsevier Inc. All rights reserved.