Cell lineage tracing reveals the plasticity of the hemocyte lineages and of the hematopoietic compartments in Drosophila melanogaster
Cell lineage tracing reveals the plasticity of the hemocyte lineages and of the hematopoietic compartments in Drosophila melanogaster
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DOI：
10.1016/j.molimm.2010.04.017
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发表时间：
2010-07-01
影响因子：
3.6
通讯作者：
Ando, Istvan
中科院分区：
文献类型：
--
作者：
Honti, Viktor;Csordas, Gabor;Ando, Istvan
我们关于造血、造血区室、造血细胞谱系和免疫的大部分知识都源自对脊椎动物免疫系统的研究。昆虫复杂的先天免疫、系统发育保守性和果蝇遗传学的力量使得我们能够研究果蝇中的免疫细胞（血细胞）谱系关系。果蝇中血细胞谱系的发育是细胞内细胞精确调控演替的结果。和细胞间事件，尽管这些相互作用的性质和程度尚不清楚。我们在这里描述了一个细胞谱系追踪系统，该系统用于分析血细胞谱系和功能不同的血细胞亚群的发育。该系统使我们能够区分两个主要的胚胎血细胞谱系，即 crq 和 Dot 谱系，它们位于两个物理上分离的区室（胚胎巨噬细胞和胚胎淋巴腺）中。我们在幼虫造血区室的构建以及细胞介导的免疫反应（封装反应）过程中跟踪了这些谱系的命运和发育。我们的结果揭示了造血区室和血细胞谱系在果蝇先天免疫系统的发育和细胞介导的免疫反应过程中具有相当大的可塑性和协同作用。 (C) 2010 Elsevier Ltd. 保留所有权利。
Much of our knowledge on hematopoiesis, hematopoietic compartments, hematopoietic cell lineages and immunity has been derived from studies on the vertebrate immune system The sophisticated innate immunity of insects, the phylogenetic conservation and the power of Drosophila genetics allowed the investigation of immune cell (hemocyte) lineage relationships in Drosophila melanogaster The development of the hemocyte lineages in Drosophila is a result of a precisely regulated succession of intracellular and intercellular events, though the nature and extent of these interactions are not known. We describe here a cell lineage tracing system set up to analyze the development of hemocyte lineages and functionally distinct hemocyte subsets. This system allowed us to distinguish two major embryonic hemocyte lineages, the crq and Dot lineages, in two, physically separated compartments, the embryonic macrophages and the embryonic lymph gland We followed the fate and development of these lineages in the construction of the larval hematopoietic compartments and during the cell-mediated immune response, the encapsulation reaction. Our results revealed the considerable plasticity and concerted action of the hematopoietic compartments and the hemocyte lineages in the development of the innate Immune system and in the course of the cell-mediated immune response in Drosophila. (C) 2010 Elsevier Ltd. All rights reserved.