Early proliferative events following intestinal resection in the rat.
Early proliferative events following intestinal resection in the rat.
复制标题
大鼠肠切除后的早期增殖事件。
DOI：
10.1097/00005176-199508000-00007
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发表时间：
1995
影响因子：
2.9
通讯作者：
Barnard,JA
中科院分区：
文献类型：
--
作者：
Sacks,AI;Warwick,GJ;Barnard,JA
小肠部分切除后，剩余的小肠粘膜代偿性增生和适应。控制增殖反应的分子机制尚不清楚，与增殖相关的事件发生的时间也未充分确定，特别是在切除术后不久。我们设计的实验，以表征早期（24小时内）增殖事件与近端肠切除术，并试图确定细胞类型，首先响应增殖刺激。对21日龄雄性Sprague-道利大鼠进行70%近端肠切除或横断（对照）。从远端（回肠）残留物中分离Poly（A）RNA。北方印迹显示，在切除后1 - 3 h，立即早期基因zif-268、nup-475和c-myc 1 -3 h有明显诱导，但在横断后没有。回肠隐窝上皮细胞中c-myc表达的免疫组织化学分析显示双相诱导，在切除后6 h最明显，在切除后24 h不太明显。5-溴脱氧尿苷（5-BrdU）的免疫染色仅限于回肠隐窝核，并在切除后24 h达到最大。所有这些事件都是在没有营养摄入的情况下观察到的。总之，这些数据表明，肠上皮细胞增殖的有效营养非依赖性刺激发生在部分小肠切除术后几分钟内，并且该刺激的第一个目标是残留肠中的隐窝上皮细胞。
SummaryPartial resection of the small intestine results in compensatory proliferation and adaptation in the remaining small intestinal mucosa. The molecular mechanisms governing the proliferative response are not known, nor has the timing of events associated with proliferation been adequately defined, particularly during the period just after resection. We designed experiments to characterize early (within 24 h) proliferative events associated with proximal intestinal resection and sought to determine the cell type that first responds to proliferative stimuli. Twenty‐one day old male Sprague‐Dawley rats underwent a 70% proximal intestinal resection or transection (control). Poly(A) RNA was isolated from the distal (ileal) remnants. Northern blots showed a marked induction of the immediate early genes zif‐268, nup‐475, andc‐myc1–3 h following resection, but not following transection. Immunohistochemical analysis ofc‐mycexpression in ileal crypt epithelial cells showed a biphasic induction that was most marked 6 h after resection and less prominent 24 h after resection. Immunostaining with 5‐bromodeoxyuridine (5‐BrdU) was restricted to ileal crypt nuclei and was maximal 24 h after resection. All these events were observed in the absence of nutrient intake. Taken together, these data show that a potent nutrient‐independent stimulus for intestinal epithelial cell proliferation occurs within minutes of partial small intestinal resection and that the first targets of this stimulus are crypt epithelial cells in the residual intestine.