miR-6086 inhibits ovarian cancer angiogenesis by downregulating the OC2/VEGFA/EGFL6 axis
miR-6086 inhibits ovarian cancer angiogenesis by downregulating the OC2/VEGFA/EGFL6 axis
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DOI：
10.1038/s41419-020-2501-5
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Ning Deng
中科院分区：
文献类型：
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作者：
Binhua Wu;Ligang Zhang;Yunfei Yu;Tongyi Lu;Yinmei Zhang;Wenhui Zhu;Qifang Song;Chengding Lv;Jiaying Guo;Yiqiao Tian;Ning Deng
miRNAs have emerged as a pivotal component of gene regulatory networks, mediating .cytokines secretion, cell cycle and differentiation regulation. However, how miRNAs collaborate .with transcription factors and downstream effector proteins that determine the fate of ovarian .cancer cells remains to be understood, especially regarding to mechanism of tumor angiogenesis .regulation. Based on the qRT-PCR and IHC analysis, we found that miR-6086 was maintained a .very low level both in ovarian cancer cell lines and tissues. Further, we identified OC2 and .EGFL6 as the direct targets of miR-6086 by luciferase assay and we observed an inverse .relationship between the expression of miR-6086 and the OC2/VEGFA/EGFL6 axis. The Western .blotting analysis suggested that OC2 could directly up-regulate VEGFA and indirectly up-regulate .EGFL6 through VEGFA. Moreover, miR-6086 could indirectly down-regulate VEGFA through .OC2. Additionally, miR-6086, siOC2 and siEGFL6 could negatively regulate the tumor growth .and angiogenesis of ovarian cancer (Skov3) in the animal studies, with the inhibition rates of .77.07%, 69.89% and 73.62%, respectively (**p <0.01). Moreover, the tumor cell proliferation, .migration, and invasion of ovarian cancer cell lines (Caov3 and Skov3) and vascular formation .(HUVECs) were significantly suppressed in vitro, by decreasing the AKT/MAPK pathways (*p .<0.05). Taken together, our results reveal that miR-6086 can suppress the angiogenesis networks .in ovarian cancer by down-regulating the OC2/VEGFA/EGFL6 axis, directly or indirectly, which .may provide potential targets for tumor therapeutics.
