The ability of surface characteristics of materials to trigger leukocyte tissue factor expression
The ability of surface characteristics of materials to trigger leukocyte tissue factor expression
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DOI：
10.1016/j.biomaterials.2009.12.016
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发表时间：
2010-03-01
期刊：
影响因子：
14
通讯作者：
Werner, Carsten
中科院分区：
文献类型：
--
作者：
Fischer, Marion;Sperling, Claudia;Werner, Carsten
生物材料诱导的血栓形成通常归因于接触相引发的血液凝固和血小板相关反应。考虑到外源性引发在体内血液凝固中的主要作用，我们通过研究表面性质与组织因子（TF）表达的相关性来研究该途径的材料相关诱导，凝血的外源途径的关键引发剂我们将材料与显示不同比例的-CH 3，-OH，白细胞中TF-mRNA的转录显示出与表面性质有关的明显差异，并随时间增加。此外，TF转录与其在白细胞上的存在、粒细胞活化、并且发现补体激活在材料接触后显示最高TF表达的细胞具有显著较低的细胞内TF，指向先前的TF释放然而，在我们的全血孵育设置的条件下，在有限的时间范围内，观察到的外源性途径的启动没有触发血液凝固（C）2009爱思唯尔有限公司版权所有。
Biomaterial-induced thrombosis IS Usually attributed to blood coagulation initiated by contact phase and platelet-related reactions Considering the major role of extrinsic initiation in blood Coagulation in vivo, we studied the material related-induction of this pathway by investigating the relevance of surface properties for the expression of Tissue Factor (TF), a critical initiator of the extrinsic pathway of coagulation We incubated materials with self-assembled monolayers of alkylthiols (SAMS) displaying various ratios of -CH3, -OH, and -CCOH terminations with fresh heparinized whole human blood in vitro The transcription of TF-mRNA in leukocytes showed clear differences in relation to Surface properties and increased over time In addition, a positive correlation between TF transcription and its presence on leukocytes, granulocyte activation, and complement activation was found Cells displaying the highest TF expression after material contact had significantly lower intracellular TF, pointing to previous TF release Yet under the conditions Of our whole blood incubation set-up within the limited time frame the observed initiation of the extrinsic pathway did not trigger blood coagulation (C) 2009 Elsevier Ltd All rights reserved.