Long-term deposition of inhaled antigen in lung resident CD11b-CD11c+ cells
Long-term deposition of inhaled antigen in lung resident CD11b-CD11c+ cells
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DOI：
10.1165/rcmb.2006-0330oc
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发表时间：
2007-04-01
影响因子：
6.4
通讯作者：
Ronchese, Franca
中科院分区：
文献类型：
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作者：
Matthews, Kate E.;Karabeg, Adela;Ronchese, Franca
在这项研究中，我们报告了一个群体的肺居民CD 11b（-）CD 11 c（+）细胞，能够采取吸入抗原和保留它的延长时间的特性。卵清蛋白-异硫氰酸荧光素结合物鼻内给予小鼠的FITC-卵清蛋白（FITC-OVA）被肺中的两个主要细胞群摄取，一个是由树突状细胞（DC）组成的迁移性CD 11 c（+）CD 11b（+）群，它将抗原迅速转运到引流淋巴结（LN），以及在给药后长达8周内保留吞噬抗原而不明显降解抗原的常驻CD 11b（-）CD 11 c（+）群体。FITC（+）CD 11b（-）CD 11 c（+）细胞没有以可检测的速率迁移到引流LN，并且没有上调共刺激分子对LIPS治疗的反应。在肺和支气管肺泡灌洗液中发现FITC（+）CD 11b（-）CD 11 c（+）细胞，它们的分布与巨噬细胞一致。尽管FITC（+）CD 11b（-）CD 11 c（+）细胞表达DC标记物DEC 205和其他与抗原呈递细胞功能相关的分子，但它们在体外不诱导抗原特异性CD 4（+）T细胞增殖或在体内不诱导活化的CD 4（+）T细胞产生急性细胞因子。因此，FITC（+）CD 11b（-）CD 11 c（+）细胞似乎代表与DC和巨噬细胞共享特性的中间细胞类型。这些细胞可能在调节肺部驻留T细胞对吸入抗原的反应中发挥作用。
In this study we report the characterization of a population of lung resident CD11b(-)CD11c(+) cells that are able to take up inhaled antigen and retain it for extended periods of time. Ovalbumin conjugated to fluorescein-isothiocyanate (FITC-OVA) administered intranasally to mice was taken up by two main populations of cells in the lung, a migratory CD11c(+)CD11b(+) population consisting of dendritic cells (DC), which rapidly transported antigen to the draining lymph node (LN), and a resident CD11b(-)CD11c(+) population that retained engulfed antigen without apparently degrading it for up to 8 wk after administration. The FITC(+)CD11b(-)CD11c(+) cells did not migrate to draining LN at a detectable rate, and did not upregulate expression of costimulatory molecules in response to LIPS treatment. FITC(+)CD11b(-)CD11c(+) cells were found in the lung and bronchoalveolar lavage fluid, and their distribution was compatible with macrophages. Although FITC(+)CD11b(-)CD11c(+) cells expressed the DC marker DEC205 and other molecules associated with antigen-presenting cell function, they did not induce proliferation of antigen-specific CD4(+) T cells in vitro or acute cytokine production by activated CD4(+) T cells in vivo. Thus, FITC(+)CD11b(-)CD11c(+) cells appear to represent an intermediate cell type sharing properties with DC and macrophages. These cells may have a role in modulating the responses of lung resident T cells to inhaled antigens.