Bacterial Flora in the Digestive Tracts of Marine Fish-III
Bacterial Flora in the Digestive Tracts of Marine Fish-III
复制标题
海水鱼消化道中的细菌菌群-III
DOI：
10.2331/suisan.38.633
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
Y. Ishida
中科院分区：
文献类型：
--
作者：
H. Sera;Y. Ishida
研究了从海水鱼消化道分离的土著和其他细菌群的一些代表性菌株对胃中遇到的环境条件（即低pH值和胃液）的反应。结果表明：（1）在pH4.0条件下培养5小时，对土著菌群（I-a1和I-a11）和IV菌群的存活率影响不大，但在相同条件下，其它菌群的存活率明显下降。一般而言，在pH 4.0下孵育时，细胞悬液的浊度未显著降低。(2)将真鲷胃液加入pH4.0的菌悬液中，培养5 h后，I-d和VI-b组菌株的活力明显下降。在pH 4.0的胃液存在下，还观察到III、VI-b和VII组菌株的浊度显著降低。胃蛋白酶、溶菌酶和胃液均不降低其活力。(3)土著细菌和VI-b群的菌株在pH 5.0下生长良好，而其他菌株在此条件下不生长。这些事实表明，土著细菌群体（I-a1和I-a11）对海洋鱼类胃中的环境条件具有独特的抗性或适应性。
The response of some representative strains of the indigenous and the other bacterial groups isolated from the digestive tracts of marine fish to the environmental conditions to be encountered in the stomach, i.e. low pH value and gastric juice, was examined. The results obtained are summarized as follows: (1) In the cases of the indigenous bacterial groups (I-a1 and I-a11) and IV group, viability was not much influenced by 5 hour's incubation at pH 4.0; however, remarkable loss in viability was observed in the other groups under the same conditions. The turbidity of cell suspensions, in general, did not decrease markedly on incubation at pH 4.0. (2) When gastric juice prepared from red sea bream was added to the pH 4.0 suspension, the viability of the strains of I-d and VI-b groups was significantly decreased after 5 hours' incubation. A marked decrease in turbidity was also observed with the strains of III, VI-b and VII groups, in the presence of gastric juice at pH 4.0. Pepsin, lysozyme and gastric juice from gizzard shad did not decrease the viability. (3) The strains of the indigenous bacterial and VI-b groups grew well at pH 5.0, while the others did not grow under this condition. These facts suggest that the indigenous bacterial groups (I-a1 and I-a11) are uniquely resistant or adapted to the environmental conditions in the stomach of marine fish.