Production and characterization of monoclonal antibodies to human sclerostin.
Production and characterization of monoclonal antibodies to human sclerostin.
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人硬化素单克隆抗体的生产和表征。
DOI：
10.1089/hyb.2009.0036
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Kumar,Rajiv
中科院分区：
文献类型：
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作者：
Craig,TheodoreA;Sommer,StacyL;Beito,ThomasG;Kumar,Rajiv
我们开发并表征了针对人和小鼠硬化素 (scl) 氨基末端和羧基末端区域的单克隆抗体。使用 f-moc 化学合成与含有 scl 结构域的 Protein-1 具有有限同源性的氨基末端和羧基末端 scl 肽。将肽与匙孔血蓝蛋白缀合，并将缀合物用于小鼠的免疫。使用细菌表达和昆虫细胞表达的重组 scl 获得并表征单克隆抗体。氨基端 (IgG 2aK) 和羧基端 (IgG 2bK) 抗体结合生物活性硬化蛋白，该硬化蛋白在昆虫细胞表达系统中表达，解离常数在纳摩尔范围内。这些抗体是可用于调节硬化素生物活性的潜在有用试剂。
We developed and characterized monoclonal antibodies directed against the amino-terminal and carboxy-terminal regions of human and mouse sclerostin (scl). Amino-terminal and carboxy-terminal scl peptides with limited homology to scl domain-containing protein-1 were synthesized using f-moc chemistry. The peptides were conjugated to keyhole limpet hemocyanin and the conjugates were used for immunization of mice. Monoclonal antibodies were obtained and characterized using bacterially expressed and insect cell–expressed recombinant scl. The amino-terminal (IgG 2aK) and carboxy-terminal (IgG 2bK) antibodies bound bioactive sclerostin that was expressed in an insect-cell expression system with dissociation constants in the nanomolar range. The antibodies are potentially useful agents that can be used for modulating sclerostin bioactivity.