Soft x-ray microscopy
Soft x-ray microscopy
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DOI：
10.1016/s0962-8924(98)01424-x
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发表时间：
1999-02-01
影响因子：
19
通讯作者：
Jacobsen, C
中科院分区：
文献类型：
--
作者：
Jacobsen, C
软X射线显微镜开始提供信息来补充从光学和电子显微镜获得的信息。软X射线显微镜可以提供30纳米分辨率的水合细胞图像，厚度类似于10微米，并且：正在努力获得更高的分辨率。虽然除了单次曝光外，活标本不容易研究，但固定标本可以高分辨率成像，快速冷冻标本可以在不进行化学修饰和不造成重大辐射损害的情况下进行研究。层析成像技术正被开发用于3D成像，光谱显微镜提供了独特的能力，可以在金标记物和荧光标记物之外，对未标记的结构进行生化测绘。目前，大多数软x-my显微镜都在同步辐射设备上工作；但实验室规模的显微镜也在开发中。
Soft x-ray microscopes are beginning to provide information to complement that obtained from optical and electron microscopy. Soft x-ray microscopy can deliver 30-nm resolution images of hydrated cells up to similar to 10 microns thick, and: efforts towards obtaining higher resolution are under way. Although living specimens cannot be studied readily except in single exposures, fixed samples can be imaged at high resolution, and flash-frozen specimens can be studied without chemical modification and without significant radiation damage. Tomography is being developed for 3-D imaging, and spectromicroscopy offers unique capabilities for biochemical mapping of unlabelled structures beyond those of gold and fluorescent labels. Currently, most soft x-my microscopes operate at synchrotron radiation-facilities; but laboratory-scale microscopes are being developed too.