Thickening of the infarcted wall by collagen injection improves left ventricular function in rats
Thickening of the infarcted wall by collagen injection improves left ventricular function in rats
复制标题
DOI：
10.1016/j.jacc.2005.04.056
复制
发表时间：
2005-08-16
影响因子：
24
通讯作者：
Kloner, RA
中科院分区：
文献类型：
--
作者：
Dai, WD;Wold, LE;Kloner, RA
结果我们确定了胶原蛋白植入是否可以使梗死的左心室（LV）壁增厚并改善LV功能。背景我们假设，通过使用胶原蛋白增厚梗死壁可能会导致一些益处，这些益处与以前报道的当梗死壁增厚细胞时的益处相似。方法将患有1周龄心肌梗死的Fischer大鼠注射胶原蛋白或盐水（100 μ l）注入瘢痕（每组n = 12）。6周后，对左室血管造影、血流动力学和局部心肌血流量进行评估。结果：与生理盐水处理组（440 +/- 34 μ m，p = 2.6 × 10（6））相比，胶原蛋白注射显著增加了瘢痕厚度（719 +/- 26 μ m）。通过左心室血管造影，胶原蛋白治疗组的每搏输出量明显更大（163 +/-μ l）比生理盐水处理组（129 +/- 6 μ l，p = 0.005），胶原治疗组的左心室射血分数也更高（48.4 +/-1.8%）比生理盐水治疗组（40.7 +/-1.0%，p = 0.002）。局部室壁运动分析显示，10只盐水处理大鼠中有5只出现反常收缩期膨出，平均为LV舒张周长的20.3 +/-2.6%，但11只胶原蛋白处理大鼠中没有（P = 0.012）。生理盐水治疗组的左心室舒张末期和收缩末期容积分别为319 +/- 12 μ l和190 +/- 7 μ l。有较大的LV舒张末期容积（343 - 23 μ L），但较小的收缩末期容积（180 - 16 μ L）在胶原蛋白治疗group.CONCLUSIONS这项研究表明，胶原蛋白注射增厚梗死瘢痕，提高LV每搏输出量和射血分数的趋势，并防止心肌梗死后矛盾的收缩期膨出。(c)2005年，美国心脏病学会基金会。
OBJECTIVES We determined whether collagen implantation could thicken the infarcted left ventricular (LV) wall and improve LV function.BACKGROUND We hypothesized that thickening the infarcted wall by using collagen might result in some benefits that are similar to what previously had been reported when the infarcted wall was thickened with cells.METHODS Fischer rats with one-week-old myocardial infarcts were injected with collagen or saline (100 mu l) into the scar (n = 12 each group). Six weeks later, LV angiograms, hemodynamics, and regional myocardial blood flow were assessed. The hearts, were processed for measurements of postmortem LV volume and histology.RESULTS Collagen injection significantly increased scar thickness (719 +/- 26 mu m) compared with the saline-treated group (440 +/- 34 mu m, p = 2.6 X 10(6)). By LV angiography, stroke volume was significantly larger in the collagen-treated group (163 +/- mu l) than in the saline-treated group (129 +/- 6 mu l, p = 0.005), and LV ejection fraction was also greater in the collagen-treated group (48.4 +/- 1.8 %) than in the saline-treated group (40.7 +/- 1.0 %, p = 0.002). Analysis of regional wall motion demonstrated paradoxical systolic bulging in 5 of 10 saline-treated rats that averaged 20.3 +/- 2.6 % of the LV diastolic circumference, but in none of the 11 collagen-treated rats (P = 0.012). The LV end-diastolic and end-systolic volumes were 319 +/- 12 mu l and 190 +/- 7 mu l in the saline-treated group, respectively. There was a trend for larger LV end-diastolic volumes (343 23 mu l), but smaller end-systolic volumes (180 16 mu l) in the collagen-treated group.CONCLUSIONS This study shows that collagen injection thickens an infarct scar and improves LV stroke volume and ejection fraction, and prevents paradoxical systolic bulging after myocardial infarction. (c) 2005 by the American College of Cardiology Foundation.