Development of ic-ELISA and lateral-flow immunochromatographic assay strip for the detection of folic acid in energy drinks and milk samples
Development of ic-ELISA and lateral-flow immunochromatographic assay strip for the detection of folic acid in energy drinks and milk samples
复制标题
DOI：
10.1080/09540105.2016.1183600
复制
发表时间：
2016-05
影响因子：
3
通讯作者：
Dezhao Kong;Liqiang Liu;Shanshan Song;Hua Kuang;Chuanlai Xu
中科院分区：
文献类型：
--
作者：
Dezhao Kong;Liqiang Liu;Shanshan Song;Hua Kuang;Chuanlai Xu
【摘要】：叶酸(FA)是人体生长发育的重要维生素,尤其是孕妇。需要一种灵敏、快速、准确的FA检测方法来评估食品的营养质量和安全性。制备了针对 FA 的单克隆抗体，用于开发间接竞争酶联免疫吸附测定 (ic-ELISA) 和侧流免疫层析测定 (ICA) 试纸条。 ic-ELISA的50%抑制浓度和检测限分别为0.12和0.018 ng/ml。侧流 ICA 条的视觉检测限和截止值分别为 0.5 和 2.5 ng/ml。使用 ICA 试纸条，能量饮料中的 FA 回收率为 89-98%，牛奶样品中的 FA 回收率为 73-87%，与传统微生物检测方法获得的结果非常一致。我们开发的方法灵敏、方便、有效，适合食品样品的现场检测和快速大规模筛查。
ABSTRACT Folic acid (FA) is an important vitamin for human growth and development, especially for pregnant women. A sensitive, rapid, and accurate FA detection method is required to assess the nutritional quality and safety of foods. A monoclonal antibody against FA was prepared for the development of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and lateral-flow immunochromatographic assay (ICA) strip. The 50% inhibitory concentration and limit of detection of ic-ELISA were 0.12 and 0.018 ng/ml, respectively. The visual limit of detection and cut-off values of the lateral-flow ICA strip were 0.5 and 2.5 ng/ml, respectively. Using the ICA strip, FA recovery rates were 89–98% from energy drinks and 73–87% for milk samples and were in good agreement with those obtained from the conventional microbiological assay method. Our developed methods are sensitive, convenient, effective, and suitable for on-site detection and rapid mass screening of food samples.