Aquaporins of the PIP2 class are required for efficient anther dehiscence in tobacco
Aquaporins of the PIP2 class are required for efficient anther dehiscence in tobacco
复制标题
DOI：
10.1104/pp.104.056408
复制
发表时间：
2005-03-01
期刊：
影响因子：
7.4
通讯作者：
Mariani, C
中科院分区：
文献类型：
--
作者：
Bots, M;Vergeldt, F;Mariani, C
高等植物有性生殖过程中的几个过程涉及水在细胞或组织之间的运动。开花前，花粉囊和花粉在开裂释放成熟花粉之前发生脱水。水通道蛋白代表一类介导水在细胞膜上运动的蛋白质。质膜PIP2家族的水通道蛋白在烟草花药中表达，因此可能参与该器官中的水运动。为了更深入地了解这些蛋白质在这一过程中可能发挥的作用，我们使用免疫定位分析了它们的定位，并生成了显示PIP2水通道蛋白RNA干扰的植物。我们的研究结果表明，PIP2蛋白的表达在花药发育过程中受到调节。此外，在烟草PIP2 RNA干扰植物，花药脱水较慢，开裂发生后，与对照植物相比。总之，我们的研究结果表明，水通道蛋白的PIP2类所需的有效花药脱水之前开裂。
Several processes during sexual reproduction in higher plants involve the movement of water between cells or tissues. Before flower anthesis, anther and pollen dehydration takes place before the release of mature pollen at dehiscence. Aquaporins represent a class of proteins that mediates the movement of water over cellular membranes. Aquaporins of the plasmamembrane PIP2 family are expressed in tobacco (Nicotiana tabacum) anthers and may therefore be involved in the movement of water in this organ. To gain more insight into the role these proteins may play in this process, we have analyzed their localization using immunolocalizations and generated plants displaying RNA interference of PIP2 aquaporins. Our results indicate that PIP2 protein expression is modulated during anther development. Furthermore, in tobacco PIP2 RNA interference plants, anther dehydration was slower, and dehiscence occurred later when compared with control plants. Together, our results suggest that aquaporins of the PIP2 class are required for efficient anther dehydration prior to dehiscence.