Methods for studying oogenesis
Methods for studying oogenesis
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DOI：
10.1016/j.ymeth.2014.01.005
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发表时间：
2014-06-15
期刊：
影响因子：
4.8
通讯作者：
Cooley, Lynn
中科院分区：
文献类型：
--
作者：
Hudson, Andrew M.;Cooley, Lynn
果蝇卵子发生是研究发育细胞生物学的一个极好的系统。活跃的研究领域包括干细胞维持、配子发育、模式形成、细胞骨架调节、细胞间通讯、细胞间运输、细胞极性、细胞迁移、细胞死亡、形态发生、细胞周期控制等等。卵室的大尺寸和相对简单的组织使它们非常适合活的和固定的整个组织的显微镜检查。卵子发生研究有许多工具可供使用。新获得的shRNA转基因系提供了经典功能丧失F2筛选和克隆筛选的替代方案。可以使用Ga 14/UAS系统在生殖系或体细胞中特异性地控制基因表达。蛋白质捕获线提供内源性水平表达的蛋白质的荧光标签，用于实时成像和筛选背景。本文综述了卵子发生研究中常用的试剂和关键方法。(C)2014 Elsevier Inc. All rights reserved.
Drosophila oogenesis is an excellent system for the study of developmental cell biology. Active areas of research include stem cell maintenance, gamete development, pattern formation, cytoskeletal regulation, intercellular communication, intercellular transport, cell polarity, cell migration, cell death, morphogenesis, cell cycle control, and many more. The large size and relatively simple organization of egg chambers make them ideally suited for microscopy of both living and fixed whole mount tissue. A wide range of tools is available for oogenesis research. Newly available shRNA transgenic lines provide an alternative to classic loss-of-function F2 screens and clonal screens. Gene expression can be specifically controlled in either germline or somatic cells using the Ga14/UAS system. Protein trap lines provide fluorescent tags of proteins expressed at endogenous levels for live imaging and screening backgrounds. This review provides information on many available reagents and key methods for research in oogenesis. (C) 2014 Elsevier Inc. All rights reserved.