SEQUENCE SPECIFICITY OF QUINOXALINE ANTIBIOTICS .2. NMR-STUDIES OF THE BINDING OF [N-MECYS(3),N-MECYS(7)]TANDEM AND TRIOSTIN-A TO DNA CONTAINING A CPI STEP
SEQUENCE SPECIFICITY OF QUINOXALINE ANTIBIOTICS .2. NMR-STUDIES OF THE BINDING OF [N-MECYS(3),N-MECYS(7)]TANDEM AND TRIOSTIN-A TO DNA CONTAINING A CPI STEP
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DOI：
10.1021/bi00207a006
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发表时间：
1994-10-18
期刊：
影响因子：
2.9
通讯作者：
FEIGON, J
中科院分区：
文献类型：
--
作者：
ADDESS, KJ;FEIGON, J
CysMeTANDEM和triostin A的DNA含有CpI步骤的结合已被研究的一维和二维H-1 NMR光谱。CysMeTANDEM作为双嵌入剂与DNA小沟中的肽骨架序列特异性结合CpI步骤以及TpA步骤。只有非特异性，nonintercalative结合之间观察到triostin A和DNA含有CpI步骤。CysMeTANDEM-[d（GGACITCC）]（2）复合物与CysMeTANDEM-[d（GGATATCC）]（2）复合物的比较表明，两种复合物的结构非常相似。然而，CysMeTANDEM与[d（GGACITCC）]（2）的结合不如与[d（GGATATCC）]（2）的结合紧密。所提出的NMR证据提供了对CysMeTANDEM与TpA位点和triostin A与CpG位点的序列特异性结合中药物与DNA之间的堆积相互作用和氢键作用的分子见解。
The binding of CysMeTANDEM and triostin A to DNA containing a CpI step has been studied by one- and two-dimensional H-1 NMR spectroscopy. CysMeTANDEM binds sequence specifically to CpI steps as well as TpA steps as a bis-intercalator with the peptide backbone in the minor groove of the DNA. Only nonspecific, nonintercalative binding is observed between triostin A and DNA containing a CpI step. Comparison of the CysMeTANDEM-[d(GGACITCC)](2) complex to the CysMeTANDEM-[d(GGATATCC)](2) complex indicates that the structures of both complexes are very similar. However, CysMeTANDEM binds less tightly to [d(GGACITCC)](2) than to [d(GGATATCC)](2). The NMR evidence presented provides molecular insight into the role of stacking interactions and hydrogen bonding between the drug and the DNA in the sequence-specific binding of CysMeTANDEM to TpA sites and of triostin A to CpG sites.