A conserved protein disulfide isomerase enhances plant resistance against herbivores
A conserved protein disulfide isomerase enhances plant resistance against herbivores
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DOI：
10.1093/plphys/kiac489
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发表时间：
2022-11-03
期刊：
影响因子：
7.4
通讯作者：
Hong, Xiao-Yue
中科院分区：
文献类型：
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作者：
Cui, Jia-Rong;Bing, Xiao-Li;Hong, Xiao-Yue
食草动物相关分子模式（HAMPs）使植物能够识别食草动物，并可能帮助植物调整其防御反应。在这里，我们报告广泛分布在节肢动物的蛋白质二硫键异构酶（PDI）的草食动物引起的变化。来自叶螨伊氏叶螨（Tetranychus evansi，TePDI）的PDI，一种全世界范围内的以叶肉为食的农业害虫，在多种茄科植物中引发免疫。TePDI介导的本氏烟草细胞死亡需要植物信号蛋白SGT1（skp1的G2等位基因的抑制因子）和热休克蛋白90（热休克蛋白90），但被蜘蛛螨效应因子Te28和Te84抑制。此外，PDIs从遗传上不同的草食性和非草食性节肢动物引发植物免疫。最后，尽管PDI诱导的植物防御损害了叶螨在植物上的表现，但RNAi实验表明PDI基因对螨和粉虱的生存至关重要。我们的研究结果表明，植物识别进化上保守的HAMPs激活植物防御和抵抗害虫伤害，指出广谱害虫管理的机会。植物识别蛋白质二硫键异构酶激活防御反应和抵抗害虫伤害。
Herbivore-associated molecular patterns (HAMPs) enable plants to recognize herbivores and may help plants adjust their defense responses. Here, we report on herbivore-induced changes in a protein disulfide isomerase (PDI) widely distributed across arthropods. PDI from the spider mite Tetranychus evansi (TePDI), a mesophyll-feeding agricultural pest worldwide, triggered immunity in multiple Solanaceae plants. TePDI-mediated cell death in Nicotiana benthamiana required the plant signaling proteins SGT1 (suppressor of the G2 allele of skp1) and HSP90 (heat shock protein 90), but was suppressed by spider mite effectors Te28 and Te84. Moreover, PDIs from phylogenetically distinct herbivorous and nonherbivorous arthropods triggered plant immunity. Finally, although PDI-induced plant defenses impaired the performance of spider mites on plants, RNAi experiments revealed that PDI genes are essential for the survival of mites and whiteflies. Our findings indicate that plants recognize evolutionarily conserved HAMPs to activate plant defense and resist pest damage, pointing to opportunities for broad-spectrum pest management.Plants recognize protein disulfide isomerase to activate defense responses and resist pest damage.