Surface alterations of mammalian cells upon ionizing radiation as detected by a lectin-binding technique. I. Binding of concanavalin A by blood cells of X-irradiated mice.
Surface alterations of mammalian cells upon ionizing radiation as detected by a lectin-binding technique. I. Binding of concanavalin A by blood cells of X-irradiated mice.
复制标题
通过凝集素结合技术检测电离辐射后哺乳动物细胞的表面变化。
DOI：
10.1080/09553008114551051
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
G. Köteles
中科院分区：
文献类型：
--
作者：
T. Kubasova;L. Varga;G. Köteles
剂量范围为0.25- 5戈伊的X射线照射诱导小鼠血细胞质膜的非常早期的剂量依赖性和暂时的功能改变，通过凝集素（伴刀豆球蛋白A）结合技术检测。在0.45- 4.5戈伊X射线照射后的前3小时内，照射红细胞与~ 3 H-伴刀豆球蛋白A的结合程度高于对照组。淋巴细胞在0.45和0.9戈伊之间也与增加的结合反应，但在2.26和4.5戈伊下观察到显著降低。血小板被证明是最敏感的。1戈伊照射后，总血小板群体和分离的“年轻”细胞亚群的结合放射性水平分别增加了4倍以上。“年轻”血小板的质膜的功能刺激发生在2戈伊以下，而结合到“老年”细胞的凝集素的量仅在2戈伊以上的剂量增加。
X-irradiation in the dose range 0.25--5 Gy induces very early dose-dependent and temporary functional alterations in the plasma membranes of murine blood cells detected by a lectin (concanavalin A)-binding technique. The irradiated erythrocytes bind 3H-concanavalin A during the first 3 hours after 0.45--4.5 Gy X-irradiation to a greater extent than the controls. The lymphocytes also react with an increased binding between 0.45 and 0.9 Gy, but a remarkable decrease is observed with 2.26 and 4.5 Gy. The platelets proved to be the most sensitive. The levels of bound radioactivity for the total platelet population and for the separated subpopulation of 'young' cells were respectively increased by a factor of four and more, after 1 Gy irradiation. The functional stimulation of plasma membranes of 'young' platelets occurred below 2 Gy, while the amount of lectin bound to 'aged' cells only increased at doses above 2 Gy.