Passive spatial and temporal integration of excitatory synaptic inputs in cerebellar Purkinje cells of young rats
Passive spatial and temporal integration of excitatory synaptic inputs in cerebellar Purkinje cells of young rats
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DOI：
10.1016/s0304-3940(03)00132-0
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发表时间：
2003-04-24
影响因子：
2.5
通讯作者：
Antkowiak, B
中科院分区：
文献类型：
--
作者：
Heck, D;Borst, A;Antkowiak, B
我们已经调查了兴奋性（平行纤维）突触输入的整合在小脑浦肯野细胞的年轻大鼠在体外，并在一个房室模型，这样的细胞，基于三维形态重建。兴奋性突触输入在两个独立的树突网站被激活的电刺激与两个刺激之间的各种延迟。当两个树突状输入位点在空间上分离（> 200 μ m）时，群体突触后电位在电流钳条件下线性相加，但当输入位点彼此接近（< 50 μ m）时，群体突触后电位以延迟依赖的方式亚线性相加。在电压钳条件下测量的群体突触后电流的总和与输入的空间和时间分离线性无关。浦肯野细胞的被动房室模型中的输入的总和与体外浦肯野细胞相似。我们发现，次线性求和的相邻输入是独立的抑制机制，并建议，次线性主要是由于本地减少驱动力。(C)2003爱思唯尔科学爱尔兰有限公司保留所有权利。
We have investigated the integration of excitatory (parallel fiber) synaptic inputs in cerebellar Purkinje cells of young rats in vitro and in a compartmental model of such a cell, based on 3D morphological reconstruction. Excitatory synaptic inputs at two independent dendritic sites were activated by electrical stimulation with various delays between the two stimuli. Population postsynaptic potentials summed linearly under current clamp condition when the two dendritic input sites were spatially separated (> 200 mum) but sublinearly, in a delay dependent manner, when the input sites were close (< 50 mum) to each other. Population postsynaptic currents measured under voltage clamp conditions summed linearly independent of the spatial and temporal separation of inputs. Summation of inputs in a passive compartmental model of a Purkinje cell was similar to that of Purkinje cells in vitro. We show that sublinear summation of neighboring inputs is independent of inhibitory mechanisms and suggest that sublinearity is mainly due to a locally reduced driving force. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.