Induction of Reactive Oxygen Species by Human T-Cell Leukemia Virus Type 1 Tax Correlates with DNA Damage and Expression of Cellular Senescence Marker
Induction of Reactive Oxygen Species by Human T-Cell Leukemia Virus Type 1 Tax Correlates with DNA Damage and Expression of Cellular Senescence Marker
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DOI：
10.1128/jvi.02460-09
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发表时间：
2010-05-01
影响因子：
5.4
通讯作者：
Jeang, Kuan-Teh
中科院分区：
文献类型：
--
作者：
Kinjo, Takao;Ham-Terhune, Julia;Jeang, Kuan-Teh
人类T细胞白血病病毒1型（HTLV-1）Tax影响细胞基因组稳定性和衰老。到目前为止，由税收引起的这些事件的机制还不完全清楚。在这里，我们表明Tax在原代人类细胞中的表达诱导活性氧（ROS），从而导致DNA损伤和衰老标志物的表达。用ROS清除剂处理或通过小干扰RNA（siRNA）敲低Tax表达消除Tax诱导的DNA损伤和衰老标记物的表达。我们的数据表明，ROS诱导解释了Tax诱导的细胞DNA损伤和细胞衰老。
Human T-cell leukemia virus type 1 (HTLV-1) Tax affects cellular genomic stability and senescence. As yet, the mechanism(s) for these events caused by Tax is incompletely understood. Here, we show that Tax expression in primary human cells induces reactive oxygen species (ROS), which elicits DNA damage and the expression of senescence marker. Treatment with a ROS scavenger or knockdown of Tax expression by small interfering RNA (siRNA) abrogated Tax-induced DNA damage and the expression of senescence marker. Our data suggest that ROS induction explains Tax-induced cellular DNA damage and cellular senescence.