Conformational requirements for activity of salmon calcitonin.
Conformational requirements for activity of salmon calcitonin.
复制标题
鲑鱼降钙素活性的构象要求。
DOI：
10.1210/endo-117-3-801
复制
发表时间：
1985
期刊：
影响因子：
4.8
通讯作者：
J. K. Seyler
中科院分区：
文献类型：
--
作者：
D. Findlay;V. Michelangeli;T. Martin;R. Orlowski;J. K. Seyler
A series of deletion-substitution analogs of salmon calcitonin (SCT) have been prepared containing combinations of a glycine substitution in position 8 and deletions of serine-2 and tyrosine-22. Biological activity of the analogs with respect to native SCT were determined in the rat hypocalcemic assay and by studying stimulation of cAMP formation and competition for binding of 125I-labeled SCT in T47 D human breast cancer cells. It was found that each of the analogs retained full potency, irrespective of the means of assessment. It is suggested that conservation of the alpha-helical region of SCT, along with the overall tertiary structure, are more important for peptide potency than chain length per se.
