Rapid increases in cytokinin concentration in lateral buds of chickpea (Cicer arietinum L.) during release of apical dominance
Rapid increases in cytokinin concentration in lateral buds of chickpea (Cicer arietinum L.) during release of apical dominance
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DOI：
10.1007/s004250050128
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发表时间：
1997
期刊：
影响因子：
4.3
通讯作者：
C. Turnbull;Myriam A. A. Raymond-Myriam-A.-A.-Raymond-49952195;I. Dodd;S. Morris
中科院分区：
文献类型：
--
作者：
C. Turnbull;Myriam A. A. Raymond-Myriam-A.-A.-Raymond-49952195;I. Dodd;S. Morris
我们研究了细胞分裂素（CKs）在鹰嘴豆侧芽顶端优势释放中的作用。芽去顶或应用CK（苄基腺嘌呤，玉米素或二氢玉米素）刺激芽的快速生长。延时录像显示，生长启动2小时内的应用程序的200 pmol苄基腺嘌呤或3小时内的断头。芽内源CK含量在去顶后2h内变化不大，但在6h后显著增加，稍晚于芽的开始生长。CK主要是玉米素核苷，其含量在6h时增加了7倍，在24h时增加了25倍。玉米素和异戊烯基腺嘌呤CK的变化较小。我们还没有区分这些CKs是否是进口的根通过木质部流或原位合成的芽，但CKs可能是一个内源性信号的一部分，参与侧芽生长刺激后，拍摄断头。据我们所知，这是第一个详细的报告CK水平在芽本身在顶端优势释放。
We examined the role of cytokinins (CKs) in release of apical dominance in lateral buds of chickpea (Cicer arietinumL.). Shoot decapitation or application of CKs (benzyladenine, zeatin or dihydrozeatin) stimulated rapid bud growth. Time-lapse video recording revealed growth initiation within 2 h of application of 200 pmol benzyladenine or within 3 h of decapitation. Endogenous CK content in buds changed little in the first 2 h after shoot decapitation, but significantly increased by 6 h, somewhat later than the initiation of bud growth. The main elevated CK was zeatin riboside, whose content per bud increased 7-fold by 6 h and 25-fold by 24 h. Lesser changes were found in amounts of zeatin and isopentenyl adenine CKs. We have yet to distinguish whether these CKs are imported from the roots via the xylem stream or are synthesised in situ in the buds, but CKs may be part of an endogenous signal involved in lateral bud growth stimulation following shoot decapitation. To our knowledge, this is the first detailed report of CK levels in buds themselves during release of apical dominance.