CARBON DIOXIDE FIXATION AND CARBOXYDISMUTASE IN THIOBACILLUS NOVELLUS
CARBON DIOXIDE FIXATION AND CARBOXYDISMUTASE IN THIOBACILLUS NOVELLUS
复制标题
DOI：
10.1016/0006-291x(65)90610-8
复制
发表时间：
1965-01-01
影响因子：
3.1
通讯作者：
HUANG, E
中科院分区：
文献类型：
--
作者：
ALEEM, MIH;HUANG, E
方法在Visbniac和Santer（1957）描述的培养基中，在自养或异养条件下培养新型硫杆菌。按照Aleem（1965）的描述制备无细胞浸提液。利用先前在敏捷硝化杆菌中使用的技术获得CO2固定和固定产物（Aleem，1965）。实验细节在适当的表格中给出。13ZSulliisandDiscussion. nuvellus生长的自养催化活性CO2固定（表I）。在添加的硫柳汞存在下发生碳同化的显著刺激，表明硫酸盐氧化被耦合以产生能量和CO2的吸能还原所必需的还原。
METHODS Thiobacillus novellus was grown under autotrophic or heterotrophic conditions in culture media described by Visbniac and Santer (1957). The cell-free extracts were prepared as described by Aleem (1965). The fixation of CO2 and products of fixation were obtained involving techniques used previously in case of Nitrobacter agilis (Aleem, 1965). The experimental details are given in appropriate tables. l3ZSuLLIIS ANDDISCUSSION Washed intact cells of z. nuvellus grown autotrophically catalyzed active CO2 fixation (Table I). A marked stimulation in the carbon assimilation occurred in the presence of added thiosdfate indicating sulfate oxidation was coupled to generate energy and reducing necessary for the endergonic reduction of C02.