Identification of a point mutation in the voltage-gated sodium channel gene of Kenyan Anopheles gambiae associated with resistance to DDT and pyrethroids
Identification of a point mutation in the voltage-gated sodium channel gene of Kenyan Anopheles gambiae associated with resistance to DDT and pyrethroids
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DOI：
10.1046/j.1365-2583.2000.00209.x
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发表时间：
2000-10-01
影响因子：
2.6
通讯作者：
Collins, FH
中科院分区：
文献类型：
--
作者：
Ranson, H;Jensen, B;Collins, FH
1990年在肯尼亚西部开始了一项氯菊酯浸渍蚊帐和窗帘的实地试验，1992年从该地点移殖了一种对氯菊酯敏感性降低的冈比亚按蚊。电压门控钠通道1014位的亮氨酸-苯丙氨酸取代与许多昆虫物种对氯菊酯和滴滴涕的抗性有关，包括西非的冈比亚按蚊。我们克隆和测序的部分钠通道的cDNA从肯尼亚氯菊酯抗性菌株，我们确定了一个替代取代（亮氨酸丝氨酸）在同一位置，这是与遗传的氯菊酯抗性的F-2后代之间的易感和耐药个体的遗传杂交。Martinez-Torres等人[（1998）Insect Mol Biol 7：179-184]开发了诊断聚合酶链反应（PCR）来检测安氏按蚊野外种群中的kdr等位基因。gambiae不会检测到肯尼亚等位基因，因此依赖该检测可能导致低估该种属中拟除虫菊酯抗性的流行率。我们采用了诊断PCR检测亮氨酸-丝氨酸突变，并与此诊断，我们能够证明，这kdr等位基因是存在于1986年从肯尼亚试验地点收集的个人，在引进拟除虫菊酯浸渍蚊帐。安。冈比亚钠离子通道定位于染色体2L，20 C区。这个位置对应于一个主要的数量性状基因座的位置，决定在肯尼亚株的安氯菊酯抗性。冈比亚。
A field trial of permethrin-impregnated bednets and curtains was initiated in Western Kenya in 1990, and a strain of Anopheles gambiae showing reduced susceptibility to permethrin was colonized from this site in 1992. A leucine-phenylalanine substitution at position 1014 of the voltage-gated sodium channel is associated with resistance to permethrin and DDT in many insect species, including Anopheles gambiae from West Africa. We cloned and sequenced a partial sodium channel cDNA from the Kenyan permethrin-resistant strain and we identified an alternative substitution (leucine to serine) at the same position, which is linked to the inheritance of permethrin resistance in the F-2 progeny of genetic crosses between susceptible and resistant individuals. The diagnostic polymerase chain reaction (PCR) developed by Martinez-Torres et al. [(1998) Insect Mol Biol 7: 179-184] to detect kdr alleles in field populations of An. gambiae will not detect the Kenyan allele and hence reliance on this assay may lead to an underestimate of the prevalence of pyrethroid resistance in this species. We adapted the diagnostic PCR to detect the leucine-serine mutation and with this diagnostic we were able to demonstrate that this kdr allele was present in individuals collected from the Kenyan trial site in 1986, prior to the introduction of pyrethroid-impregnated bednets. The An. gambiae sodium channel was physically mapped to chromosome 2L, division 20C. This position corresponds to the location of a major quantitative trait locus determining resistance to permethrin in the Kenyan strain of An. gambiae.