Allogeneic anorectal transplantation in rats: technical considerations and preliminary results.
Allogeneic anorectal transplantation in rats: technical considerations and preliminary results.
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DOI：
10.1038/srep30894
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发表时间：
2016-08-04
影响因子：
4.6
通讯作者：
D'Albuquerque LC
中科院分区：
文献类型：
--
作者：
Galvão FH;Waisberg DR;Seid VE;Costa AC;Chaib E;Baptista RR;Capelozzi VL;Lanchotte C;Cruz RJ;Araki J;D'Albuquerque LC
大便失禁是一种具有挑战性的疾病，有多种可用的治疗方式。这些方法的成功率各不相同，当失败时通常需要进行永久性结肠造口术。对于这些病例，一种新的潜在治疗策略是肛门直肠移植（ATx）。我们进行了四次同基因（Lewis-to-Lewis）和七次同种异体（Wistar-to-Lewis）ATx 手术。用包含与肠系膜下动脉连续的主动脉和与肠系膜下静脉连续的门静脉的血管蒂回收肛门直肠。在受者中，切除原生肛门直肠段，并通过端侧主动脉-主动脉和门静脉吻合术以及端对端结直肠吻合术移植移植物。在术后第 30 天的实验终点处死受体。存活的动物在手术后 5 天内恢复正常的体重增加和临床表现。同种移植物和 42.9% 的同种异体移植物达到了正常的临床进化直至实验终点。 57.1%的同种异体移植物在移植后三周观察到免疫排斥症状（腹胀、腹泻和肛门粘膜炎症）。组织学显示同种异体移植物中存在中度至重度排斥反应，而同种移植物中没有排斥迹象。我们描述了一种可行的大鼠 ATx 模型，这可能允许进一步的生理学和免疫学研究。
Fecal incontinence is a challenging condition with numerous available treatment modalities. Success rates vary across these modalities, and permanent colostomy is often indicated when they fail. For these cases, a novel potential therapeutic strategy is anorectal transplantation (ATx). We performed four isogeneic (Lewis-to-Lewis) and seven allogeneic (Wistar-to-Lewis) ATx procedures. The anorectum was retrieved with a vascular pedicle containing the aorta in continuity with the inferior mesenteric artery and portal vein in continuity with the inferior mesenteric vein. In the recipient, the native anorectal segment was removed and the graft was transplanted by end-to-side aorta-aorta and porto-cava anastomoses and end-to-end colorectal anastomosis. Recipients were sacrificed at the experimental endpoint on postoperative day 30. Surviving animals resumed normal body weight gain and clinical performance within 5 days of surgery. Isografts and 42.9% of allografts achieved normal clinical evolution up to the experimental endpoint. In 57.1% of allografts, signs of immunological rejection (abdominal distention, diarrhea, and anal mucosa inflammation) were observed three weeks after transplantation. Histology revealed moderate to severe rejection in allografts and no signs of rejection in isografts. We describe a feasible model of ATx in rats, which may allow further physiological and immunologic studies.
影响因子：
4.6
作者：
Araki J;Nishizawa Y;Nakamura T;Sato T;Naito M;Hatayama N;Hirai S;Tashiro K;Koshima I
通讯作者：
Koshima I