DNA synthesis and ploidy in non-Hodgkin's lymphomas demonstrate intrapatient variation depending on circadian stage of cell sampling.
DNA synthesis and ploidy in non-Hodgkin's lymphomas demonstrate intrapatient variation depending on circadian stage of cell sampling.
复制标题
非霍奇金淋巴瘤中的 DNA 合成和倍性表现出患者体内的差异，具体取决于细胞采样的昼夜节律阶段。
作者：
R. Smaaland;Knut Lote;Robert B. Sothern;Ole Didrik Laerum
在24例组织学上已确定的非霍奇金淋巴瘤患者（18男6女）中发现了显著的昼夜节律细胞周期变化，夜间s期细胞数量最多。采用细针穿刺法每隔4 h穿刺抽吸26例患者的病理淋巴结，每隔24 h进行一次抽吸。24例（92.3%）为III期或IV期疾病。根据工作配方，低级别淋巴瘤12例（46.1%），中级淋巴瘤10例（38.5%），高级别淋巴瘤4例（15.4%）。采用流式细胞术对样品进行分析，并根据昼夜节律测定DNA合成（s期）和倍性。个体平均24小时s期从2.2 +/- 1.2%（平均+/- SD）到24.0 +/- 3.3%不等。在低级别淋巴瘤患者组中，平均s期范围从2.4 +/- 1.2%到9.2 +/- 2.8%。与24小时内的最低值（变化范围）相比，每个患者最低和最高s期之间的百分比变化从21%到353%不等，平均变化范围为128 +/- 19%。当将每个个体s期序列转换为平均值的百分比，并通过单向方差分析进行分析，以检验2个12小时时间跨度（晚上8点至上午8点与上午8点至晚上8点）的时间效应时，发现昼夜节律阶段的s期变化具有统计学意义（P < 0.004），其中8点至上午8点时间跨度的s期变化值更高。通过单余弦分析，对于24小时余弦对所有数据的最小二乘拟合（占平均值的百分比），s相产生了接近显著的P值0.069，其中顶相发现在午夜附近（0.05小时）。对于那些平均s期值< 10.0%的中低级别淋巴瘤患者，我们发现冬季（5.8 +/- 0.4%）的平均s期高于春季（3.8 +/- 0.3%）或秋季（3.6 +/- 0.3%）（P < 0.001，方差分析）。26例患者中有21例（80.8%）在一个或多个重复样本中存在非整倍体、次二倍体或近二倍体群体。非整倍体群体的DNA指数变化范围为1.09 ~ 1.96，中位数为1.20。（摘要删节为400字）
Significant circadian cell cycle variations with a maximal number of cells in S-phase during the night have been found in a series of 24 patients (18 men and 6 women) with histologically established non-Hodgkin's lymphomas. Pathological lymph nodes of a total of 26 patients were punctured and aspirated by fine needle technique every 4 h during a single 24-h time span. Twenty-four patients (92.3%) had Stage III or IV disease. Twelve patients (46.1%) had low grade, 10 patients (38.5%) had intermediate grade, and 4 patients (15.4%) had high grade lymphomas according to the Working Formulation. The samples were analyzed by flow cytometry, and DNA synthesis (S-phase) and ploidy were determined according to circadian stage. The individual mean 24-h S-phase varied from 2.2 +/- 1.2% (mean +/- SD) to 24.0 +/- 3.3%. Within the group of patients with low grade lymphomas, a wide range in mean S-phase from 2.4 +/- 1.2% to 9.2 +/- 2.8% was observed. The percentage variation within each patient between the lowest and highest S-phase as compared to the lowest value (range of change) during the 24-h time span varied from 21 to 353%, with a mean range of change of 128 +/- 19%. When each individual S-phase series was converted to percent of mean and combined for analysis by one-way analysis of variance to test for time-effect across 2 12-h time spans (8 p.m.-8 a.m. versus 8 a.m.-8 p.m.), S-phase variation according to circadian stage was found to be statistically significant (P < 0.004), with higher values found in the 8 p.m.-8 a.m. time span. By single cosinor analysis, S-phase yielded a near significant P value of 0.069 for the least-squares fit of a 24-h cosine to all data as percent of mean, with the acrophase found to be near midnight (0.05 h). For those patients with low and intermediate grade lymphomas and with mean S-phase values < 10.0%, we found that mean S-phase was higher during winter (5.8 +/- 0.4%) than during spring (3.8 +/- 0.3%) or during fall (3.6 +/- 0.3%) (P < 0.001, analysis of variance). Twenty-one of the 26 patients (80.8%) had an aneuploid, hypodiploid, or near diploid population in one or several of the repeated samples. For the whole series, the DNA indices for the aneuploid populations varied from 1.09 to 1.96, the median DNA index being 1.20.(ABSTRACT TRUNCATED AT 400 WORDS)
非霍奇金淋巴瘤的标志物和动力学研究。
DOI：
10.1016/0002-9343(81)90265-5
发表时间：
1981
期刊：
The American journal of medicine
影响因子：
--
作者：
Hansen,H;Koziner,B;Clarkson,B
通讯作者：
Clarkson,B
影响因子：
3.8
作者：
Tewes, Mitra;Aktas, Bahriye;Kasimir-Bauer, Sabine
通讯作者：
Kasimir-Bauer, Sabine