ROLE OF ANGIOGENESIS IN PATIENTS WITH CEREBRAL ISCHEMIC STROKE
ROLE OF ANGIOGENESIS IN PATIENTS WITH CEREBRAL ISCHEMIC STROKE
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DOI：
10.1161/01.str.25.9.1794
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发表时间：
1994-09-01
期刊：
影响因子：
8.3
通讯作者：
WANG, JM
中科院分区：
文献类型：
--
作者：
KRUPINSKI, J;KALUZA, J;WANG, JM
背景和目的中风是西方世界最常见的死亡和发病原因之一。它是由脑动脉闭塞引起的，随后通过微血管向脑组织的血液供应严重紊乱。尽管有大量的文献，其病理生理学是知之甚少，这严重阻碍了逻辑发展的therapy.Methods脑，从10例年龄在46至85岁的生存时间为5至92天后，他们中风。收集对侧未受累脑半球的切除区域和代表性对照组织。显微镜下测量微血管密度。在10801个区域中共对6520个微血管进行评分。使用三种单克隆抗体，即E-9，针对活化的内皮细胞提出的免疫组化研究内皮细胞的活化水平; IG 11，识别血管细胞粘附分子-1;和抗增殖细胞核抗原。组织提取物中的血管生成活性进行了检查，使用在体内鸡绒毛尿囊膜assay.Results的微血管（Wilcoxon对数秩检验; P小于或等于0.01）的数量有统计学意义的增加，在9个10个梗死脑组织时，与他们的对侧正常半球。斯皮尔曼rho分析证实，在这些患者中，较高的血管计数与较长的生存期相关（P
Background and Purpose Stroke is one of the most common causes of mortality and morbidity in the Western world. It results from the occlusion of a cerebral artery followed by severe disturbances in blood supply through microvessels to brain tissue. Despite an extensive literature its pathophysiology is poorly understood, and this has severely impeded the logical development of therapy.Methods Brains were obtained from 10 patients aged 46 to 85 years with survival times of 5 to 92 days after their stroke. Infarcted areas and representative control tissues from the contralateral uninvolved brain hemisphere were collected. Microvessel density was measured microscopically. A total of 6520 microvessels were scored in 10 801 areas. The level of activation of the endothelial cells was studied by immunohistochemistry using three monoclonal antibodies, viz, E-9, raised against activated endothelial cells; IG11, recognizing vascular cell adhesion molecule-1; and anti-proliferating cell nuclear antigen. Angiogenic activity in tissue extracts was examined using an in vivo chicken chorioallantoic membrane assay.Results There was a statistically significant increase in the number of microvessels (Wilcoxon log-rank test; P less than or equal to.01) in 9 of 10 infarcted brain tissues when compared with their contralateral normal hemisphere. In these patients the higher blood vessel counts correlated with longer survival, as ascertained by Spearman's rho analysis (P