The Relative Rates of Iodination of Serum Components and the Effect of Iodination on Antibody Activity1
The Relative Rates of Iodination of Serum Components and the Effect of Iodination on Antibody Activity1
复制标题
血清成分的相对碘化率以及碘化对抗体活性的影响1
DOI：
10.1021/ja01161a098
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发表时间：
1950
影响因子：
15
通讯作者：
L. Sternberger
中科院分区：
文献类型：
--
作者：
D. Pressman;L. Sternberger
免疫化学方法在测定蛋白质混合物中单一蛋白质组分方面具有独特的灵敏度，可用于研究各种蛋白质反应的动力学。可以测量各种试剂与单个纯蛋白质的反应动力学。然而，通过免疫化学方法可以分析一种蛋白质的变化率相对于同一系统中其他蛋白质的变化率。这种相对反应速度的测量的优点是，混合在一起的各种蛋白质的反应性的比较必须在相同的条件下进行，因此不存在单独进行单个蛋白质的反应时可能存在的不可控变量。用蛋白质特异性抗体来分离单个蛋白质可以确定各种蛋白质的相对改变，或者用特定抗原来分离改变的抗体来确定抗体的相对改变。由于我们的研究组对组织抗体的碘化很感兴趣，所以我们进行了这次调查，以确定抗体与其他血清成分的相对反应速率常数6，以及碘化对抗体活性的影响。蛋白质的碘化作用已经得到了广泛的研究，众所周知，碘可能附着在酪氨酸残基上，也可能附着在组氨酸残基上，并能氧化蛋白质分子的各个部分。7a抗血清的碘化首先是由Blinl和Hauro-witz7b进行的，他们发现，随着碘化程度的增加，马抗血清对副伤寒A、副伤寒和马匹的凝集力降低。
Immunochemical methods are unique in their sensitivity for the determination of a single protein component in a mixture of proteins, and can be useful in thestudy of the kinetics of various protein reactions. The reaction kinetics of various reagents with individual pure proteins can be measured. 4 However, it is possible by immuno-chemical methods to analyze the rate of alteration of one protein relative to the rates of alteration of other proteins in the same system. Such measure-ments of relative rates of reaction have the ad-vantage that the comparison of the reactivities of the various proteins, which are present together in a mixture, must undergo reaction under identi-cal conditions and thus there are no uncontrolled variables which may exist when the reactions of the individual proteins are run separately. The relative alteration of various proteins can be determined by using the protein specific antibody to isolate the individual proteins; or the rela-tive alteration of the antibody can be determined by using the specific antigen to isolate the altered antibody.Since our research group has been interestedin the iodination of antibodies to tissues, 5 we carried out the present investigation to determine the relative reaction rate constant6 for iodination of antibody and other serum components, and the effect of iodination on antibody activity. The iodination of proteins has been rather widely studied and it is known that iodine probably attaches itself to the tyrosine rests and perhaps thehistidine rests, and can oxidize various parts of the protein molecule. 7a The iodination of antisera was first carried out by Breinl and Hauro-witz7b who showed that increasing degrees of iodination decreased the agglutinating power ofhorse antisera specific toward paratyphoid A, para-