The nucleoporin Nup358 associates with and regulates interphase microtubules
The nucleoporin Nup358 associates with and regulates interphase microtubules
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DOI：
10.1016/j.febslet.2007.11.087
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发表时间：
2008-01-01
期刊：
影响因子：
3.5
通讯作者：
Dasso, Mary
中科院分区：
文献类型：
--
作者：
Joseph, Jomon;Dasso, Mary
核孔蛋白Nup358位于间期核孔复合体(NPC)的细胞质表面。在中期，它重新聚集到动粒，对于正确的微管-动粒连接是重要的。在这里，我们报道了一小部分内源性Nup358通过其N-末端区域(BPN)与间期微管相互作用。过度表达Nup358的微管靶向结构域的细胞在微管组织中发生了显着的变化，包括微管束的增加和稳定性。异位表达的BPN和全长Nup358显示出显著更高水平的乙酰化微管，这些微管对微管解聚剂诺可达唑具有耐药性。此外，RNAi介导的Nup358的缺失影响了细胞定向迁移过程中微管的极化稳定，证实了Nup358在体内对间期微管的调节作用。(C)2007年欧洲生化学会联合会。爱思唯尔出版，版权所有。
The nucleoporin Nup358 resides on the cytoplasmic face of the interphase nuclear pore complex (NPC). During metaphase, its recruitment to kinetochores is important for correct microtubule-kinetochore attachment. Here, we report that a fraction of endogenous Nup358 interacts with interphase microtubules through its N-terminal region (BPN). Cells overexpressing the microtubule targeting domain of Nup358 displayed dramatic alteration in the microtubule organization including increased microtubule bundling and stability. Ectopic expression of BPN and full-length Nup358 exhibited significantly higher levels of acetylated microtubules that were resistant to nocodazole, a microtubule depolymerizing agent. Furthermore, RNAi mediated depletion of Nup358 affected polarized stabilization of microtubules during directed cell migration, confirming the in vivo role of Nup358 in regulating interphase microtubules. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.