Synthesis and secretion of protein by hepatocytes entrapped within calcium alginate.
Synthesis and secretion of protein by hepatocytes entrapped within calcium alginate.
复制标题
海藻酸钙内肝细胞合成和分泌蛋白质。
作者：
Y. Miura;T. Akimoto;H. Kanazawa;K. Yagi
为了开发生物人工肝，对捕获的肝细胞分泌血浆蛋白的能力进行了检查。通过用胶原酶灌注从成年大鼠肝脏中分离肝细胞。分离的肝细胞被海藻酸钙包裹。被捕获的细胞在地塞米松和二丁酰环 AMP 存在下诱导酪氨酸转氨酶 (TAT)，并保留诱导 TAT 的能力 7 天。此外，被捕获的细胞可以合成并分泌具有生物活性的凝血因子 II，即凝血酶原。两种血浆蛋白，卵磷脂：胆固醇酰基转移酶和胆碱酯酶，也被分泌到培养基中。因此，海藻酸钙中的肝细胞表现出肝脏特异性特征，并且这些活性几乎与单层培养细胞的活性相当。
The ability of entrapped hepatocytes to secrete plasma proteins was examined for the purpose of developing a biological artificial liver. Hepatocytes were isolated from adult rat liver by perfusion with collagenase. Isolated hepatocytes were entrapped within calcium alginate. The entrapped cells induced tyrosine aminotransferase (TAT) in the presence of dexamethasone and dibutyryl-cyclic AMP and retained the ability to induce TAT for 7 days. Moreover, entrapped cells could synthesize and secrete a biologically active form of coagulation Factor II, prothrombin. Two plasma proteins, lecithin: cholesterol acyltransferase and cholinesterase, were also secreted into the medium. Thus, hepatocytes within calcium alginate showed liver-specific characteristics, and these activities were almost comparable with those of monolayer-cultured cells.