Caloric restriction increases the expression of heat shock protein in the gut
Caloric restriction increases the expression of heat shock protein in the gut
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DOI：
10.1097/00000658-199605000-00015
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发表时间：
1996-05-01
影响因子：
9
通讯作者：
Thompson, JC
中科院分区：
文献类型：
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作者：
Ehrenfried, JA;Evers, BM;Thompson, JC
目的研究热量限制（CR）是否改变肠道热休克蛋白70（hsp 70）基因表达，并探讨CR对热休克蛋白70基因表达的影响。癌症）;这些影响的机制尚不清楚。热休克蛋白可吞噬一组应激反应基因，其中最主要的是热休克蛋白70。方法实验一，取成年（4月龄）大鼠（n = 3/组），分别于禁食48 h、再喂养6 h和24 h后处死，另取3只大鼠（对照组），不禁食或再喂养。取胃提取酸性RNA用于hsp 70基因的表达。在第二个实验中，老年（22- 26月龄）大鼠随意（AL）或CR饮食（AL饮食的60%热量摄入）。结果在第一次实验中，禁食48 h的大鼠胃中hsp 70 mRNA的表达量增加了约3倍，再进食后6 h和24 h，hsp 70 mRNA的表达量下降到对照组水平。在第二个实验中，热休克蛋白70的mRNA水平显着增加，在两个老年CR大鼠胃和十二指肠与AL controls.Conclusions相比，作者已经证明，热休克蛋白70的mRNA水平增加，在近端肠道的年轻和老年大鼠，无论是急性（与持久）或CR。肠道中细胞保护性hsp 70基因表达的增加可能为CR的有益作用提供了可能的细胞机制。
Objective The authors determined whether caloric restriction (CR), either acutely or chronically, alters heat shock protein 70 (hsp70) gene expression in the gut.Summary Background Data Caloric restriction prolongs the life span and delays age-related disease (e.g., cancer) in mammals; the mechanisms responsible for these effects are not known. Heat shock proteins ate a group of stress-responsive genes of which the most prominent member is hsp70.Methods In the first experiment, adult (4-month-old) rats (n = 3/group) were killed after a 48-hour fast or 6 and 24 hours after refeeding, In addition, three rats (controls) were killed without fasting or refeeding. The stomach was removed acid RNA was extracted for hsp70 gene expression. In the second experiment, aged (22- to 26-month-old) rats were fed ad libitum (AL) or a CR diet (60% caloric intake of AL diet). Rats were killed, the stomach and duodenum were removed, and RNA was extracted for determination of hsp70 gene expression.Results In the first experiment, hsp70 mRNA levels were increased approximately threefold in the stomach of rats fasted for 48 hours; levels decreased to control values by 6 and 24 hours after refeeding. In the second experiment, hsp70 mRNA levels were increased significantly in both the stomach and duodenum of aged CR rats compared with AL controls.Conclusions The authors have demonstrated that hsp70 mRNA levels are increased in the proximal gut of young and old rats, either acutely (with lasting) or with CR. Increased expression of the cytoprotective hsp70 gene in the gut may provide a possible cellular mechanism for the beneficial effects noted With CR.