Cataracts and photochemical damage in the lens.
Cataracts and photochemical damage in the lens.
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白内障和晶状体的光化学损伤。
DOI：
10.1002/9780470720875.ch6
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Borkman,RF
中科院分区：
文献类型：
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作者：
Borkman,RF
来自阳光和其他环境源的紫外线辐射被认为是某些人类白内障的主要致病因素。流行病学和实验数据的文献进行了审查和新的实验结果暴露的大鼠晶状体紫外激光辐射在体外。晶状体的这种UV照射显示引起：（i）透镜蛋白中色氨酸残基的光化学改变。导致色氨酸荧光的损失，（ii）归因于透镜蛋白的光氧化产物的新的紫外吸收带，和（iii）通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳检测的透镜蛋白中多肽的交联。值得注意的是，所报道的UV辐射诱导的透镜变化与正常人晶状体发生白内障时发现的变化非常相似。为了建立LJV暴露的晶状体类似于自然发生的白内障的程度，建议对正常、白内障和UV暴露的人类晶状体的解剖学、组织学和生物化学参数进行全面比较。
The possibility is considered that ultraviolet radiation, from sunlight and other ambient sources, is a major causative factor in certain human cataracts. Epidemiological and experimental data from the literature are reviewed and new experimental results on exposure of rat lenses to UV laser radiation in vitro are presented. This UV irradiation of lenses is shown to cause:(i) photochemical alteration of tryptophan residues in lens proteins. resulting in loss of tryptophan fluorescence,(ii) new ultraviolet absorption bands attributable to photo-oxidation products of lens proteins, and (iii) cross-linking of polypeptides in lens proteins, detected by sodium dodecylsulphate-polyacrylamide gel electrophoresis. It is noted that the UV radiation-induced lens changes reported are quite similar to the changes found when normal human lenses develop cataracts. In order to establish the degree to which LJV-exposed lenses resemble naturally occurring cataracts, a comprehensive comparison of anatomical, histological and biochemical parameters for normal, cataractous and UV-exposed human lenses is proposed.