Probing the Molecular Mechanisms for Pristinamycin Yield Enhancement in Streptomyces pristinaespiralis
Probing the Molecular Mechanisms for Pristinamycin Yield Enhancement in Streptomyces pristinaespiralis
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DOI：
10.1007/s00284-012-0233-1
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发表时间：
2012-09
影响因子：
2.6
通讯作者：
Q. Jin;Zhi-hua Jin;Lijing Zhang;Shanjing Yao;Fuyong Li
中科院分区：
文献类型：
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作者：
Q. Jin;Zhi-hua Jin;Lijing Zhang;Shanjing Yao;Fuyong Li
采用实时荧光定量PCR（Q-PCR）和扩增片段长度多态性（AFLP）技术，对基因组改组获得的普那霉素高产重组菌株进行了初步研究。Q-PCR分析表明，分别参与原始霉素II和I组分生物合成的snaB和snbA在重组子中的表达量比在原始菌株中的表达量更高，表明它们的表达变化可能是该抗生素生物合成过程中的关键因素。此外，由于抗性基因在重组子中先于抗生素产生开始而开始高水平表达，因此对原始霉素的高自身抗性的先行建立也可能导致抗生素产量的增加。对这些重组子进行AFLP分析，发现两个新基因的基因组变异，即AfsR调节基因和转座酶基因的同源物，表明这两个基因的变异可能与普那霉素产量的提高有关。本研究为提高普那霉素产量提供了一些潜在的分子线索。
The mechanisms for the enhancement of pristinamycin production in the high-yielding recombinants ofStreptomyces pristinaespiralisobtained by genome shuffling were investigated by quantitative real-time PCR (Q-PCR) and amplified fragment length polymorphism (AFLP) techniques. Q-PCR analysis showed thatsnaBandsnbAinvolved, respectively, in the biosynthesis of pristinamycins II and I component had more extended high expression in the recombinant than that in the ancestor during fermentation process, indicating their expression changes might be key factors during the biosynthesis of the antibiotic. In addition, the antecedent establishment of the high self-resistance to pristinamycin, becauseptrresistance gene started high-level expression ahead of the onset of the antibiotic production in the recombinant, might also lead to the increase of the antibiotics yield. AFLP analysis of these recombinants revealed genome variation of two novel genes, the homologs ofAfsRregulatory gene and transposase gene, indicating these two gene variations were probably responsible for yield improvement of pristinamycin. This study provided several potential molecular clues for pristinamycin yield enhancement.