Kinetics of the mucosal antibody secreting cell response and evidence of specific lymphocyte migration to the lung after oral immunisation with attenuated S-enterica var. typhimurium
Kinetics of the mucosal antibody secreting cell response and evidence of specific lymphocyte migration to the lung after oral immunisation with attenuated S-enterica var. typhimurium
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DOI：
10.1111/j.1574-695x.2000.tb01440.x
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发表时间：
2000-04-01
影响因子：
--
通讯作者：
Strugnell, RA
中科院分区：
文献类型：
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作者：
Allen, JS;Dougan, G;Strugnell, RA
由疫苗载体沙门氏菌肠变种引起的粘膜分泌反应的动力学。鼠伤寒沙门氏菌（S.用酶联免疫斑点法（ELISPOT）检测鼠伤寒沙门氏菌（Typhimurium）的血清学反应。用强毒沙门氏菌aroA、aroD突变体BRD 509口服免疫小鼠。表达破伤风毒素（TT）C片段的鼠伤寒沙门氏菌SL 1344在胃肠道固有层、脾和肺中同时产生了抗S.鼠伤寒杆菌脂多糖（LPS）和TT. ASC反应的幅度在肠道中最大，在第25天通过二次免疫加强，并且反应的动力学与血清抗体的出现无关。本研究表明，S.鼠伤寒沙门氏菌可与普通粘膜免疫系统结合，以在远端部位产生粘膜分泌应答，然而，应答的幅度在肠道和抗原特异性中均最大。血清中S.鼠伤寒沙门氏菌和由该细菌表达的抗原尚未阐明。(C)2000年，欧洲微生物学会联合会。由Elsevier Science B.V.出版，版权所有。
The kinetic of mucosal secretory responses elicited by the vaccine vector Salmonella enterica var. typhimurium (S. typhimurium) was examined by enzyme linked immunospot (ELISPOT) and compared with serum responses. Mice immunised orally with BRD509, the aroA, aroD mutant of virulent S. typhimurium SL1344 expressing the C Fragment of tetanus toxin (TT), simultaneously developed an IgA antibody secreting cells (ASC) response in the gastrointestinal lamina propria, the spleen and the lung, against both S. typhimurium lipopolysaccharide (LPS) and TT. The magnitude of the ASC response was greatest in the gut, was boosted by a secondary immunisation at day 25, and the kinetic of the response did not correlate with the appearance of serum antibodies. This study suggests that S. typhimurium can engage the common mucosal immune system to effect mucosal secretory responses at distal sites, however, the magnitude of the responses is both greatest in the gut and antigen-specific. The ASC origin of the serum antibodies specific for S. typhimurium and antigens expressed by the bacterium is yet to be elucidated. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.