A general description of detachment for multidimensional modelling of biofilms
A general description of detachment for multidimensional modelling of biofilms
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DOI：
10.1002/bit.20544
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发表时间：
2005-09-20
影响因子：
3.8
通讯作者：
van Loosdrecht, MCM
中科院分区：
文献类型：
--
作者：
Xavier, JD;Picioreanu, C;van Loosdrecht, MCM
介绍了多维生物膜建模中描述生物质脱离的通用方法。使用连续脱离速度函数 F-det 对作用于生物膜整个表面的过程（例如侵蚀）造成的生物质损失进行建模。离散脱离事件，即脱落，是从模拟中隐式导出的。该方法非常灵活，允许 F-det 采用多种形式，包括依赖于任何状态变量（例如局部生物膜密度）的表达式。通过使用水平集方法的新颖应用，将这种生物质分离方法与基于多维（2D 和 3D）颗粒的多物种生物膜模型集成。该方法的应用通过生物膜结构和活性的动态趋势来说明，该趋势源自对考虑均匀生物量的简单模型（案例研究 I）和区分异养活性物质、细胞外聚合物质（EPS）和惰性物质中的生物量组成的模型（案例研究 II）进行的模拟。案例研究 I 的结果证明了施加的分离力作为影响稳态生物膜活性和结构的基本因素的影响。文献中报告的实验观察的趋势得到了正确的描述。例如，模拟结果表明，当侵蚀力增加时，生物量脱落会减少。案例研究 II 说明了分离方法在生物质成分不均匀的系统中的应用。在不同基质浓度下进行的模拟显示，由于氧限制或基质限制生长，生物膜结构（在生物质成分的形状、密度和空间分布方面）和活性（在氧气和基质消耗方面）发生变化。 (c) 2005 年 Wiley 期刊公司。
A general method for describing biomass detachment in multidimensional biofilm modelling is introduced. Biomass losses from processes acting on the entire surface of the biofilm, such as erosion, are modelled using a continuous detachment speed function F-det. Discrete detachment events, i.e. sloughing, are implicitly derived from simulations. The method is flexible to allow F-det to take several forms, including expressions dependent on any state variables such as the local biofilm density. This methodology for biomass detachment was integrated with multidimensional (2D and 3D) particle-based multispecies biofilm models by using a novel application of the level set method. Application of the method is illustrated by trends in the dynamics of biofilms structure and activity derived from simulations performed on a simple model considering uniform biomass (case study I) and a model discriminating biomass composition in heterotrophic active mass, extracellular polymeric substances (EPS) and inert mass (case study II). Results from case study I demonstrate the effect of applied detachment forces as a fundamental factor influencing steady-state biofilm activity and structure. Trends from experimental observations reported in literature were correctly described. For example, simulation results indicated that biomass sloughing is reduced when erosion forces are increased. Case study II illustrates the application of the detachment methodology to systems with non-uniform biomass composition. Simulations carried out at different bulk concentrations of substrate show changes in biofilm structure (in terms of shape, density and spatial distribution of biomass components) and activity (in terms of oxygen and substrate consumption) as a consequence of either oxygen-limited or substrate-limited growth. (c) 2005 Wiley Periodicals, Inc.