ESTABLISHMENT AND CHARACTERIZATION OF MULTIPOTENT NEURAL CELL-LINES USING RETROVIRUS VECTOR-MEDIATED ONCOGENE TRANSFER
ESTABLISHMENT AND CHARACTERIZATION OF MULTIPOTENT NEURAL CELL-LINES USING RETROVIRUS VECTOR-MEDIATED ONCOGENE TRANSFER
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DOI：
10.1002/neu.480210209
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发表时间：
1990-03-01
期刊：
影响因子：
--
通讯作者：
CEPKO, CL
中科院分区：
文献类型：
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作者：
RYDER, EF;SNYDER, EY;CEPKO, CL
通过逆转录病毒载体介导的禽myc癌基因转导产生神经细胞系。靶细胞为出生后小鼠嗅球和小脑以及出生后大鼠大脑皮质的有丝分裂祖细胞。感染的前两个领域，其中神经发生和胶质细胞发生出生后，产生多能克隆线，表现出神经元和神经胶质细胞的表型，和一个线具有稳定的神经元表型。感染大脑皮层，其中胶质细胞，但不是神经发生，发生在出生后，产生致命的克隆，表现出胶质细胞表型的细胞。这些线应证明有价值的体外和体内研究，旨在了解控制细胞的命运和分化的神经祖细胞。
Neural cell lines were produced by retroviral vector-mediated transduction of the avian myc oncogene. Target cells were mitotic progenitor cells of postnatal mouse olfactory bulb and cerebellum, and postnatal rat cerebral cortex. Infection of the first two areas, where neurogenesis and gliogenesis occur postnatally, produced multipotent clonal lines that exhibited phenotypes of both neuronal and glial cells, and one line with a stable neuronal phenotype. Infection of cerebral cortex, where gliogenesis, but not neurogenesis, occurs postnatally, generated mortal clones that exhibited cells of glial phenotype. These lines should prove valuable for both in vitro and in vivo studies aimed at understanding the control of cell fate and differentiation of neural progenitors.