Hantavirus-like particles generated in CHO cells induce specific immune responses in C57BL/6 mice
Hantavirus-like particles generated in CHO cells induce specific immune responses in C57BL/6 mice
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DOI：
10.1016/j.vaccine.2010.04.025
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发表时间：
2010-06-11
期刊：
影响因子：
5.5
通讯作者：
Li, Dexin
中科院分区：
文献类型：
--
作者：
Li, Chuan;Liu, Feng;Li, Dexin
由于汉他病毒在世界范围内分布并引起急性且通常致命的疾病（肾综合征出血热，HFRS），因此非常需要安全有效的汉他病毒疫苗。展示功能性病毒蛋白的病毒样颗粒（VLP）可以提供针对一些病毒的有效疫苗，但其诱导汉坦病毒特异性免疫应答的能力尚未得到充分研究。为了检测汉滩病毒样颗粒（HTN-VLP）疫苗的免疫原性，我们在中国仓鼠卵巢（CHO）细胞中共表达汉滩病毒核衣壳（N）蛋白和糖蛋白（Gn和Gc），制备了重组HTN-VLP。我们比较了肌内与皮下注射HTN-VLP诱导针对汉滩病毒感染的特异性免疫应答的能力。HTN-VLP肌肉和皮下免疫小鼠，均能有效激发抗汉滩病毒N蛋白和糖蛋白的特异性抗体应答，与国产汉滩病毒双价灭活疫苗相当。此外，接种HTN-VLP也导致诱导更高水平的特异性细胞反应的N蛋白比灭活疫苗。我们的研究结果提供了一个重要的洞察发展汉坦病毒样颗粒作为一个潜在的候选疫苗的控制和预防汉坦病毒感染。(C)2010爱思唯尔有限公司版权所有。
A safe and effective hantavirus vaccine is highly desirable since hantaviruses are distributed worldwide and cause an acute and often fatal disease (hemorrhagic fever with renal syndrome, HFRS). Virus-like particles (VLPs) displaying functional viral proteins could provide effective vaccines against a few viruses, but their ability to induce hantavirus-specific immune response has not been adequately investigated. To measure the immunogenicity of Hantaan virus-like particles (HTN-VLPs) vaccine, we generated recombinant HTN-VLPs by co-expressing Hantaan virus nucleocapsid (N) protein and glycoproteins (Gn and Gc) in Chinese hamster ovary (CHO) cells. We compared intramuscular versus subcutaneous administration of HTN-VLPs for the ability to induce specific immune response against Hantaan virus infection. Mice that received both intramuscular and subcutaneous immunizations of HTN-VLPs were sufficiently stimulated specific antibody response against Hantaan virus N protein and glycoproteins, which was comparable to Chinese commercial inactivated bivalent hantaviruses vaccine. Moreover, vaccination with HTN-VLPs also resulted in the induction of higher levels of specific cellular response to N protein than that of inactivated vaccine. Our results provide an important insight towards the development of hantaviruses-like particles as a potential candidate vaccine for the control and prevention of hantaviruses infection. (C) 2010 Elsevier Ltd. All rights reserved.