Physico-chemical examination of polyglutamic acid from Bacillus anthracis grown in vivo.
Physico-chemical examination of polyglutamic acid from Bacillus anthracis grown in vivo.
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体内生长的炭疽杆菌聚谷氨酸的理化检查。
DOI：
10.1042/bj0630443
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发表时间：
1956
影响因子：
4.1
通讯作者：
R. Wallis
中科院分区：
文献类型：
--
作者：
B. R. Record;R. Wallis
研究的两种聚谷氨酸样品是由我们的同事H. T. Zwartouw和H. Smith从感染炭疽芽孢杆菌的豚鼠中分离，8如先前的论文所述（Zwartouw & Smith，1956）。这些样本为：（a）从受感染动物的血浆渗出物中获得的细胞外物质;（B）从动物血液中分离的生物体的荚膜物质。两种样品均以钠盐形式获得，含有约14- 8%的钠，并进行无盐透析和冷冻干燥。它们已在电泳装置和超离心器中检查了均匀性，并在一定浓度范围内测定了沉降和扩散系数，以估计其分子量。简要讨论了聚谷氨酸的高离子性所带来的特殊困难。
The two specimens of polyglutamic acid investigated were isolated by our colleagues H. T. Zwartouw and H. Smith from guinea pigs infected with Bacillus anthraci8, as described in a previous paper (Zwartouw & Smith, 1956). These specimens were: (a) extracellular material obtained from the plasma exudate ofthe infected animals; (b) capsular material from the organisms separated from the blood of the animals. Both specimens were obtained as sodium salts, containing about 14-8 % of sodium, and were dialysed salt-free and freeze-dried. They have been examined in the electrophoresis apparatus and the ultracentrifuge for homogeneity, and sedimentation and diffusion coefficients have been determined over a range of concentration to estimate their molecular weights. The special difficulties arising from the highly ionic nature of polyglutamic acid, a typical polyelectrolyte, are briefly discussed.