Comparison between nuclear localization of nopaline- and octopine-specific Agrobacterium VirE2 proteins in plant, yeast and mammalian cells.
Comparison between nuclear localization of nopaline- and octopine-specific Agrobacterium VirE2 proteins in plant, yeast and mammalian cells.
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DOI：
10.1046/j.1364-3703.2001.00065.x
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发表时间：
2001-05
影响因子：
4.9
通讯作者：
T. Tzfira;V. Citovsky
中科院分区：
文献类型：
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作者：
T. Tzfira;V. Citovsky
摘要在一个独特的跨王国DNA转移案例中，农杆菌通过将其DNA片段转移到宿主细胞核并将其整合到植物基因组中来遗传转化植物。这一过程中的核心角色之一是细菌毒力蛋白VirE2，它与运输的DNA分子结合，并促进其核输入。由两个主要农杆菌菌株诺帕林和章鱼碱编码的VirE2蛋白的核导入被认为是通过不同的机制发生的，即萝卜碱病毒E2只导入到植物细胞的细胞核中，而章鱼碱病毒E2也积累在动物细胞的细胞核中。在这里，通过系统地比较双子叶植物和单子叶植物以及活的哺乳动物和酵母细胞中诺帕林和章鱼碱特异的病毒E2的核进口，来检验这一概念。这些实验表明，核进口的鸦片碱和章鱼碱VirE2蛋白是植物特有的，发生在植物中，而不是在非植物系统中。
SUMMARY In a unique case of trans-kingdom DNA transfer, Agrobacterium genetically transforms plants by transferring its DNA segment into the host cell nucleus and integrating it into the plant genome. One of the central players in this process is the bacterial virulence protein, VirE2, which binds the transported DNA molecule and facilitates its nuclear import. Nuclear import of VirE2 proteins encoded by two major Agrobacterium strains, nopaline and octopine, has been hypothesized to occur by different mechanisms, i.e. the nopaline VirE2 was imported only into the nuclei of plant cells while the octopine VirE2 also accumulated in the nuclei of animal cells. Here, this notion was tested by a systematic comparison of nuclear import of nopaline- and octopine-specific VirE2 in dicotyledonous and monocotyledonous plants and in living mammalian and yeast cells. These experiments showed that nuclear import of both nopaline and octopine VirE2 proteins is plant-specific, occurring in plant but not in non-plant systems.